Knockdown of Na,K-ATPase β-subunits in Oncopeltus fasciatus induces molting problems and alterations in tracheal morphology.

Autor: Herbertz M; Division of Molecular Evolutionary Biology, Department of Biology, Institute of Cell and Systems Biology of Animals, Universität Hamburg, Hamburg, 20146, Germany., Lohr J; Division of Molecular Evolutionary Biology, Department of Biology, Institute of Cell and Systems Biology of Animals, Universität Hamburg, Hamburg, 20146, Germany., Lohr C; Division of Neurophysiology, Department of Biology, Institute of Cell and Systems Biology of Animals, Universität Hamburg, Hamburg, 20146, Germany., Dobler S; Division of Molecular Evolutionary Biology, Department of Biology, Institute of Cell and Systems Biology of Animals, Universität Hamburg, Hamburg, 20146, Germany.
Jazyk: angličtina
Zdroj: Insect science [Insect Sci] 2023 Apr; Vol. 30 (2), pp. 375-397. Date of Electronic Publication: 2022 Oct 11.
DOI: 10.1111/1744-7917.13117
Abstrakt: The ubiquitously expressed transmembrane enzyme Na,K-ATPase (NKA) is vital in maintaining functionality of cells. The association of α- and β-subunits is believed to be essential for forming a functional enzyme. In the large milkweed bug Oncopeltus fasciatus four α1-paralogs and four β-subunits exist that can associate into NKA complexes. This diversity raises the question of possible tissue-specific distribution and function. While the α1-subunits are known to modulate cardenolide-resistance and ion-transport efficiency, the functional importance of the β-subunits needed further investigation. We here characterize all four different β-subunits at the cellular, tissue, and whole organismal scales. A knockdown of different β-subunits heavily interferes with molting success resulting in strongly hampered phenotypes. The failure of ecdysis might be related to disrupted septate junction (SJ) formation, also reflected in β2-suppression-induced alteration in tracheal morphology. Our data further suggest the existence of isolated β-subunits forming homomeric or β-heteromeric complexes. This possible standalone and structure-specific distribution of the β-subunits predicts further, yet unknown pump-independent functions. The different effects caused by β knockdowns highlight the importance of the various β-subunits to fulfill tissue-specific requirements.
(© 2022 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje