The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows.

Autor: Pitta DW; Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, PA, 19348, USA. dpitta@vet.upenn.edu., Indugu N; Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, PA, 19348, USA., Melgar A; Department of Animal Science, The Pennsylvania State University, State College, PA, 16801, USA., Hristov A; Department of Animal Science, The Pennsylvania State University, State College, PA, 16801, USA., Challa K; Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, PA, 19348, USA., Vecchiarelli B; Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, PA, 19348, USA., Hennessy M; Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, PA, 19348, USA., Narayan K; Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, PA, 19348, USA., Duval S; Research Centre for Animal Nutrition and Health, DSM Nutritional Products, CH-4303, Kaiseraugst, Switzerland., Kindermann M; Research Centre for Animal Nutrition and Health, DSM Nutritional Products, CH-4303, Kaiseraugst, Switzerland., Walker N; Research Centre for Animal Nutrition and Health, DSM Nutritional Products, CH-4303, Kaiseraugst, Switzerland.
Jazyk: angličtina
Zdroj: Microbiome [Microbiome] 2022 Sep 13; Vol. 10 (1), pp. 146. Date of Electronic Publication: 2022 Sep 13.
DOI: 10.1186/s40168-022-01341-9
Abstrakt: Background: Enteric methane emissions from dairy cows are an environmental problem as well as a gross feed energy loss to the animal. Methane is generated in the rumen by methanogenic archaea from hydrogen (H 2 ) + carbon dioxide and from H 2 + methanol or methylamines. The methanogenic substrates are provided by non-methanogens during feed fermentation. Methane mitigation approaches have yielded variable results, partially due to an incomplete understanding of the contribution of hydrogenotrophic and methylotrophic archaea to methanogenesis. Research indicates that 3-nitrooxypropanol (3-NOP) reduces enteric methane formation in dairy cows by inhibiting methyl-coenzyme M reductase (MCR), the enzyme responsible for methane formation. The purpose of this study was to utilize metagenomic and metatranscriptomic approaches to investigate the effect of 3-NOP on the rumen microbiome and to determine the fate of H 2 that accumulates less than expected under inhibited methanogenesis.
Results: The inhibitor 3-NOP was more inhibitory on Methanobrevibacter species than methanol-utilizing Methanosphaera and tended to reduce the gene expression of MCR. Under inhibited methanogenesis by 3-NOP, fluctuations in H 2 concentrations were accompanied by changes in the expression of [FeFe] hydrogenases in H 2 -producing bacteria to regulate the amount of H 2 production. No previously reported alternative H 2 sinks increased under inhibited methanogenesis except for a significant increase in gene expression of enzymes involved in the butyrate pathway.
Conclusion: By taking a metatranscriptomic approach, this study provides novel insights on the contribution of methylotrophic methanogens to total methanogenesis and regulation of H 2 metabolism under normal and inhibited methanogenesis by 3-NOP in the rumen. Video Abstract.
(© 2022. The Author(s).)
Databáze: MEDLINE
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