Formation and activity of NLRP3 inflammasome and histopathological changes in the lung of corpses with COVID-19.

Autor: Gholaminejhad M; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.; Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Forouzesh M; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran., Ebrahimi B; Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Mahdavi SA; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran., Mirtorabi SD; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran., Liaghat A; Legal Medicine Research Center, Legal Medicine Organization, Mashhad, Iran., Monabati SJ; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran., Hamza MO; Department of Medical Laboratory Techniques, College of Medical Technical, Al-Farahidi University, Baghdad, Iraq., Hassanzadeh G; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran. hassanzadeh@tums.ac.ir.; Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. hassanzadeh@tums.ac.ir.; Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. hassanzadeh@tums.ac.ir.
Jazyk: angličtina
Zdroj: Journal of molecular histology [J Mol Histol] 2022 Dec; Vol. 53 (6), pp. 883-890. Date of Electronic Publication: 2022 Sep 14.
DOI: 10.1007/s10735-022-10101-w
Abstrakt: COVID-19 is a contagious disease that attacks many organs but the lungs are the main organs affected. The inflammasome activation results in the exacerbation of inflammatory response in infectious disease. The aim of this study is to investigate the formation and activity of the NLRP3 inflammasome complex and the histopathological changes caused by the coronavirus in the lung of deceased persons with COVID-19. In total, 10 corpses; 5 corpses with no history of any infectious diseases and COVID-19 and 5 corpses with the cause of death of COVID-19 were included in this study. Lung tissue samples were harvested during autopsy under safe conditions. Fresh tissues in each group were used to measure the genes expression and proteins level of NLRP3, ASC, Caspase-1, IL-1β, IL-6 and TNF-α and a routine hematoxylin and eosin staining was performed for histological assessment. Data are represented as the means ± SD. Statistical significance difference was accepted at a p-value less than 5%. The NLRP3, ASC, Caspase-1, IL-1β, IL-6 and TNF-α genes expression and proteins level were elevated in the lung of the COVID-19 group in comparison with the control group. Histological findings presented the increasing number of polymorphonuclear leukocytes, macrophages and also pulmonary fibrosis in the lungs of corpses with the cause of death of COVID-19. High expression of NLRP3 inflammasome components and its relation with the pathophysiology of the coronavirus-infected lung suggested that targeting the NLRP3 inflammasome could be helpful in achieving a more effective treatment in patients with COVID-19.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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