Natural killer cells 56 bright 16 - have higher counts in the umbilical cord blood than in the adult peripheral blood.
| Autor: | de Molla VC; Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil; Hospital 9 de Julho, São Paulo, Brazil., Barbosa MCR; Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil., Junior AM; Hospital 9 de Julho, São Paulo, Brazil., Gonçalves MV; Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil., Guirao EKF; Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil., Yamamoto M; Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil., Arrais-Rodrigues C; Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil; Hospital 9 de Julho, São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Hematology, transfusion and cell therapy [Hematol Transfus Cell Ther] 2023 Oct-Dec; Vol. 45 (4), pp. 419-427. Date of Electronic Publication: 2022 Aug 19. |
| DOI: | 10.1016/j.htct.2022.07.001 |
| Abstrakt: | Introduction and Hypothesis: Umbilical cord blood (UCB) is an alternative source of hematopoietic stem cells for allogeneic hematopoietic stem cell transplantation in the absence of a compatible donor. The UCB transplantation has a lower incidence of chronic graft versus host disease (GvHD), but is associated with slower engraftment and slower immune reconstitution, compared to other sources. Dendritic cells (DCs) and Natural Killer cells (NKs) play a central role in the development of GvHD and the graft versus leukemia (GvL) effect, as well as in the control of infectious complications. Method: We quantified by multiparametric flow cytometry monocytes, lymphocytes, NK cells, and DCs, including their subsets, in UCB samples from 54 healthy newborns and peripheral blood (PB) from 25 healthy adult volunteers. Results: In the UCB samples, there were higher counts of NK cells 56 bright 16 - (median 0.024 × 109/L), compared to the PB samples (0.012 × 109/L, p < 0.0001), NK 56 dim 16 bright (median 0.446 × 109/L vs. 0.259 × 109/L for PB samples, p = 0.001) and plasmacytoid dendritic cells (pDCs, median 0.008 × 109/L for UCB samples vs. 0.006 × 109/L for PB samples, p = 0.03). Moreover, non-classic monocyte counts were lower in UCB than in PB (median 0.024 × 109/L vs. 0.051 × 109/L, respectively, p < 0.0001). Conclusion: In conclusion, there were higher counts of NK cells and pDCs and lower counts of non-classic monocytes in UCB than in PB from healthy individuals. These findings might explain the lower incidence and severity of chronic GvHD, although maintaining the GvL effect, in UCB transplant recipients, compared to other stem cell sources. Competing Interests: Conflicts of interest There are no conflicts of interest to report. (Copyright © 2022 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier España, S.L.U. All rights reserved.) |
| Databáze: | MEDLINE |
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