Wnt11 in regulation of physiological and pathological cardiac growth.

Autor:	Halmetoja E; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Nagy I; Department of Clinical Chemistry, Cancer and Translational Medicine Research Unit, Medical Research Center, University of Oulu and Northern Finland Laboratory Centre NordLab, Oulu University Hospital, Oulu, Finland., Szabo Z; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Alakoski T; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Yrjölä R; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Vainio L; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Viitavaara E; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Lin R; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Rahtu-Korpela L; Research Unit of Biomedicine, University of Oulu, Oulu, Finland., Vainio S; Laboratory of Developmental Biology, Center for Cell Matrix Research, University of Oulu, Oulu, Finland.; Kvantum Institute, Infotech Oulu, University of Oulu, Oulu, Finland., Kerkelä R; Research Unit of Biomedicine, University of Oulu, Oulu, Finland.; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland.; Biocenter Oulu, University of Oulu, Oulu, Finland., Magga J; Research Unit of Biomedicine, University of Oulu, Oulu, Finland.; Biocenter Oulu, University of Oulu, Oulu, Finland.
Jazyk:	angličtina
Zdroj:	FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2022 Oct; Vol. 36 (10), pp. e22544.
DOI:	10.1096/fj.202101856RRRR
Abstrakt:	Wnt11 regulates early cardiac development and left ventricular compaction in the heart, but it is not known how Wnt11 regulates postnatal cardiac maturation and response to cardiac stress in the adult heart. We studied cell proliferation/maturation in postnatal and adolescent Wnt11 deficient (Wnt11-/-) heart and subjected adult mice with partial (Wnt11+/-) and complete Wnt11 (Wnt11-/-) deficiency to cardiac pressure overload. In addition, we subjected primary cardiomyocytes to recombinant Wnt proteins to study their effect on cardiomyocyte growth. Wnt11 deficiency did not affect cardiomyocyte proliferation or maturation in the postnatal or adolescent heart. However, Wnt11 deficiency led to enlarged heart phenotype that was not accompanied by significant hypertrophy of individual cardiomyocytes. Analysis of stressed adult hearts from wild-type mice showed a progressive decrease in Wnt11 expression in response to pressure overload. When studied in experimental cardiac pressure overload, Wnt11 deficiency did not exacerbate cardiac hypertrophy or remodeling and cardiac function remained identical between the genotypes. When subjecting cardiomyocytes to hypertrophic stimulus, the presence of recombinant Wnt11 together with Wnt5a reduced protein synthesis. In conclusion, Wnt11 deficiency does not affect postnatal cardiomyocyte proliferation but leads to cardiac growth. Interestingly, Wnt11 deficiency alone does not substantially modulate hypertrophic response to pressure overload in vivo. Wnt11 may require cooperation with other noncanonical Wnt proteins to regulate hypertrophic response under stress. (© 2022 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)
Databáze:	MEDLINE
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