Spectrofluorimetric determination of selected genotoxic impurities in pharmaceutical raw materials and final products.

Autor: Shallan AI; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt., Abdel-Hakim A; Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897, Egypt. ali.hassan@fop.usc.edu.eg., Hammad MA; Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897, Egypt., Abou El-Alamin MM; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt. maha_abdelrehim@pharm.helwan.edu.eg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Sep 12; Vol. 12 (1), pp. 15319. Date of Electronic Publication: 2022 Sep 12.
DOI: 10.1038/s41598-022-19603-9
Abstrakt: A green spectrofluorimetric method was introduced for the determination of selected genotoxic impurities; 2-aminopyridine and 3-aminopyridine in different pharmaceutical raw materials and dosage forms. The method relied on the native fluorescence of these impurities in acidic medium. The experimental conditions were carefully studied and optimized, and the method was validated according to International Council on Harmonisation (ICH) guidelines. The linear range for both analytes was 2.50-100 ng/mL with good determination coefficients of 0.9995 and 0.9992 and detection limits of 0.62 ng/mL and 0.74 ng/mL for 2-aminopyridine and 3-aminopyridine, respectively. The method was successfully applied for determination of 2-aminopyridine and 3-aminopyridine in four active pharmaceutical ingredients and nine dosage forms with satisfactory percentage recoveries and without interference from co-formulated excipients. Analytical performance of the proposed method was comparable to that of the reported methods; hence, the proposed method can be used as a simple and low-cost alternative in quality control laboratories.
(© 2022. The Author(s).)
Databáze: MEDLINE
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