Comparison of Diagnostic Methods for Detection of Trichomonas vaginalis in Prediagnosed Vaginitis Cases and Its Association with Various Pathogens

Autor: Turan Faraşat V; Manisa Şehir Hastanesi, Tıbbi Parazitoloji Kliniği, Manisa, Türkiye, Balcıoğlu İC; Manisa Celal Bayar Üniversitesi Tıp Fakültesi, Tıbbi Parazitoloji Anabilim Dalı, Manisa, Türkiye, Solmaz Hasdemir P; Manisa Celal Bayar Üniversitesi Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Manisa, Türkiye, Gümüş E; Manisa Şehir Hastanesi, Kadın Hastalıkları ve Doğum Kliniği, Manisa, Türkiye
Jazyk: angličtina
Zdroj: Turkiye parazitolojii dergisi [Turkiye Parazitol Derg] 2022 Sep 12; Vol. 46 (3), pp. 167-171.
DOI: 10.4274/tpd.galenos.2022.02996
Abstrakt: Objective: Parasitological diagnostic methods such as direct microscopy, staining examination and culture methods are frequently used in the diagnosis of  Trichomonas vaginalis (T. vaginalis) . Though, nowadays, new diagnostic methods, especially DNA-based methods, are developing, enabling the simultaneous recognition of different pathogens. In our study, we evaluated whether  the choice of multiplex polymerase chain reaction (PCR), in which  T. vaginalis  and different pathogens can be detected, is be an alternative to classical methods and to evaluate the possible coexistence of pathogens.
Methods: In our study, swab samples taken during routine examination of 100 female patients who presented to Manisa Celal Bayar University and Manisa City Hospital Outpatient Clinics Obstetrics and Gynecology were evaluated. The presence of  T. vaginalis  was investigated in these samples by direct microscopy, Giemsa stain and culture. Besides  T. vaginalis , other possible agents were also investigated by real-time multiplex PCR method.
Results: At least one agent was detected in 85 (85%) of the 100 patient samples included in our study.  T. vaginalis positivity was detected in 6 (6%) of the samples by parasitological diagnosis methods and in 10 (10%) of the samples by multiplex PCR. Additionally, with real-time multiplex PCR,  Chlamydia trachomatis  in 4 (4%),  Neisseria gonorrhoeae  in 3 (3%),  Ureaplasma urealyticum/parvum  in 68 (68%),  Gardnerella vaginalis  in 68 (68%) and Herpes simplex virus 1/2 in 1 (1%) of the sample positivity was found.  Mycoplasma genitalium , another agent examined by multiplex PCR, was not found positive in any sample. The Kappa value of the culture that is a parasitological test and multiplex PCR for  T. vaginalis  showed moderate agreement with 59.5%.
Conclusion: It has been concluded that using  real-time multiplex PCR method, which has  high specificity and sensitivity, in addition to microscopy and culture methods in the diagnosis of  T. vaginalis , could contribute to the correct and effective treatment by detecting multiple infections.
Databáze: MEDLINE