Bovine Lactoferrin Induces Cell Death in Human Prostate Cancer Cells.

Autor: Rocha VP; Department of Biochemistry, Biomedical Institute, UNIRIO, 20211-040, Brazil.; Cell and Molecular Biology Graduate Program, UNIRIO, 20211-040, Brazil., Campos SPC; Medical Biochemistry Institute, UFRJ, 21941-590, Brazil., Barros CA; Department of Biochemistry, Biomedical Institute, UNIRIO, 20211-040, Brazil.; Multicentre Graduate Program in Biochemistry and Molecular Biology, IFRJ, 20270-021, Brazil., Trindade P; Cell and Molecular Biology Graduate Program, UNIRIO, 20211-040, Brazil.; D'Or Institute for Research and Education-IDOR, 22281-100, Brazil., Souza LRQ; D'Or Institute for Research and Education-IDOR, 22281-100, Brazil., Silva TG; National Center for Structural Biology and Bioimaging, CENABIO, UFRJ, 21941-902, Brazil., Gimba ERP; Natural Science Department, UFF, 28895-532, Brazil.; Cellular and Molecular Oncology Program, National Institute of Cancer (INCA), 20231-050, Brazil., Teodoro AJ; Department of Nutrition and Dietetics, UFF, 22290-250, Brazil., Gonçalves RB; Department of Biochemistry, Biomedical Institute, UNIRIO, 20211-040, Brazil.; Cell and Molecular Biology Graduate Program, UNIRIO, 20211-040, Brazil.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2022 Sep 02; Vol. 2022, pp. 2187696. Date of Electronic Publication: 2022 Sep 02 (Print Publication: 2022).
DOI: 10.1155/2022/2187696
Abstrakt: Bovine lactoferrin (bLf) is a multifunctional protein widely associated with anticancer activity. Prostate cancer is the second most frequent type of cancer worldwide. This study was aimed at evaluating the influence of bLf on cell viability, cell cycle progression, reactive oxygen species (ROS) production, and rate of apoptosis in the human prostate cancer cell line (DU-145). MTT assay and trypan blue exclusion were used to analyze cell viability. Morphological changes were analyzed through optical microscopy after 24 h and 48 h of bLf treatment. FITC-bLf internalization and cellular damage were observed within 24 h by confocal fluorescence microscopy. Cell cycle analyses were performed by flow cytometry and propidium iodide. For caspases 3/7 activation and reactive oxygen species production evaluation, cells were live-imaged using the high-throughput system Operetta. The cell viability assays demonstrated that bLf induces cell death and morphological changes after 24 h and 48 h of treatment compared to control on DU-145 cells. The bLf internalization was detected in DU-145 cells, G 1 -phase arrest of the cell cycle, caspase 3/7 activation, and increased oxidative stress on bLf-treated cells. Our data support that bLf has an important anticancer activity, thus offering new perspectives in preventing and treating prostate cancer.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.
(Copyright © 2022 Vanessa P. Rocha et al.)
Databáze: MEDLINE
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