Vitamin D 3 preserves blood retinal barrier integrity in an in vitro model of diabetic retinopathy.

Autor: Lazzara F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Longo AM; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Giurdanella G; Faculty of Medicine and Surgery, University of Enna 'Kore', Enna, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Lupo G; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Platania CBM; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Rossi S; Multidisciplinary Department of Medical, Surgical and Dental Sciences, University of Campania 'Luigi Vanvitelli', Naples, Italy., Drago F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Anfuso CD; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Bucolo C; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy.
Jazyk: angličtina
Zdroj: Frontiers in pharmacology [Front Pharmacol] 2022 Aug 26; Vol. 13, pp. 971164. Date of Electronic Publication: 2022 Aug 26 (Print Publication: 2022).
DOI: 10.3389/fphar.2022.971164
Abstrakt: The impairment of the blood retinal barrier (BRB) represents one of the main features of diabetic retinopathy, a secondary microvascular complication of diabetes. Hyperglycemia is a triggering factor of vascular cells damage in diabetic retinopathy. The aim of this study was to assess the effects of vitamin D 3 on BRB protection, and to investigate its regulatory role on inflammatory pathways. We challenged human retinal endothelial cells with high glucose (HG) levels. We found that vitamin D 3 attenuates cell damage elicited by HG, maintaining cell viability and reducing the expression of inflammatory cytokines such as IL-1β and ICAM-1. Furthermore, we showed that vitamin D 3 preserved the BRB integrity as demonstrated by trans-endothelial electrical resistance, permeability assay, and cell junction morphology and quantification (ZO-1 and VE-cadherin). In conclusion this in vitro study provided new insights on the retinal protective role of vitamin D 3 , particularly as regard as the early phase of diabetic retinopathy, characterized by BRB breakdown and inflammation.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Lazzara, Longo, Giurdanella, Lupo, Platania, Rossi, Drago, Anfuso and Bucolo.)
Databáze: MEDLINE
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