Single-shot optical projection tomography for high-speed volumetric imaging of dynamic biological samples.

Autor: Darling C; Photonics Group, Department of Physics, Imperial College London, London, UK., Davis SPX; Photonics Group, Department of Physics, Imperial College London, London, UK., Kumar S; Photonics Group, Department of Physics, Imperial College London, London, UK.; Francis Crick Institute, London, UK., French PMW; Photonics Group, Department of Physics, Imperial College London, London, UK.; Francis Crick Institute, London, UK., McGinty J; Photonics Group, Department of Physics, Imperial College London, London, UK.; Francis Crick Institute, London, UK.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2023 Feb; Vol. 16 (2), pp. e202200232. Date of Electronic Publication: 2022 Sep 29.
DOI: 10.1002/jbio.202200232
Abstrakt: A single-shot adaptation of Optical Projection Tomography (OPT) for high-speed volumetric snapshot imaging of dynamic mesoscopic biological samples is presented. Conventional OPT has been applied to in vivo imaging of animal models such as D. rerio, but the sequential acquisition of projection images typically requires samples to be immobilized during the acquisition. A proof-of-principle system capable of single-shot tomography of a ~1 mm 3 volume is presented, demonstrating camera-limited rates of up to 62.5 volumes/s, which has been applied to 3D imaging of a freely swimming zebrafish embryo. This is achieved by recording eight projection views simultaneously on four low-cost CMOS cameras. With no stage required to rotate the sample, this single-shot OPT system can be implemented with a component cost of under £5000. The system design can be adapted to different sized fields of view and may be applied to a broad range of dynamic samples, including high throughput flow cytometry applied to model organisms and fluid dynamics studies.
(© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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