Effects of the bovine SLICK1 mutation in PRLR on sweat gland area, FOXA1 abundance, and global gene expression in skin.

Autor:	Sosa F; Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville 32611-0910., Carmickle AT; Department of Animal Science, University of California-Davis, Davis 95616., Oliveira LJ; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602., Sagheer M; Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville 32611-0910., Saleem M; Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville 32611-0910; Department of Theriogenology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan., Yu FH; Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville 32610., Altman MD; Department of Animal Science, University of California-Davis, Davis 95616., Dikmen S; Faculty of Veterinary Medicine, Department of Animal Science, University of Uludag, Bursa, 16059, Turkey., Denicol AC; Department of Animal Science, University of California-Davis, Davis 95616., Sonstegard TS; Acceligen, Eagan, MN 55121., Larson CC; Okeechobee County Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Okeechobee 34972., Hansen PJ; Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville 32611-0910. Electronic address: pjhansen@ufl.edu.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2022 Nov; Vol. 105 (11), pp. 9206-9215. Date of Electronic Publication: 2022 Sep 07.
DOI:	10.3168/jds.2022-22272
Abstrakt:	The SLICK1 mutation in the prolactin receptor (PRLR) results in a short-hair coat and increased ability to regulate body temperature during heat stress. It is unclear whether the mutation affects capacity for sweating. The objective of this observational study was to evaluate whether the SLICK1 mutation in PRLR alters characteristics of skin related to sweat gland abundance or function. Skin biopsies from 31 Holstein heifers, including 14 wild-type (SL -/- ) and 17 heterozygous slick (SL +/- ), were subjected to histological analysis to determine the percent of the surface area of skin sections that are occupied by sweat glands. We detected no effect of genotype on this variable. Immunohistochemical analysis of the forkhead transcription factor A1 (FOXA1), a protein essential for sweating in mice, from 6 SL -/- and 6 SL +/- heifers indicated twice as much FOXA1 in sweat glandular epithelia of SL +/- heifers as in SL -/- heifers. Results from RNA sequencing of skin biopsies from 5 SL -/- and 7 SL +/- heifers revealed few genes that were differentially expressed and none that have been associated with sweat gland development or function. In conclusion, results do not support the idea that the SLICK1 mutation changes the abundance of sweat glands in skin, but do show that functional properties of sweat glands, as indicated by increased abundance of immunoreactive FOXA1, are modified by inheritance of the mutation in PRLR. (The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)
Databáze:	MEDLINE
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