Fluorescent indicators for imaging membrane potential of organelles.

Autor: Klier PEZ; Department of Chemistry, University of California, Berkeley, CA, 94720, USA., Roo R; Department of Chemistry, University of California, Berkeley, CA, 94720, USA., Miller EW; Department of Chemistry, University of California, Berkeley, CA, 94720, USA; Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA, 94720, USA. Electronic address: evanwmiller@berkeley.edu.
Jazyk: angličtina
Zdroj: Current opinion in chemical biology [Curr Opin Chem Biol] 2022 Dec; Vol. 71, pp. 102203. Date of Electronic Publication: 2022 Sep 06.
DOI: 10.1016/j.cbpa.2022.102203
Abstrakt: Plasma membrane potential is a key driver of the physiology of excitable cells like neurons and cardiomyocytes. Voltage-sensitive fluorescent indicators offer a powerful complement to traditional electrode-based approaches to measuring and monitoring membrane potential. Intracellular organelles can also generate membrane potential, yet the electrode- and fluorescent indicator-based approaches used for plasma membrane potential imaging are difficult to implement on intact organelles in their native environment. Here, we survey recent advances in developing and deploying voltage-sensitive fluorescent indicators to interrogate organelle membrane potential in intact cells.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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