High-Throughput Binder Confirmation (HTBC).
| Autor: | Xia B; Encoded Library Technologies/NCE Molecular Discovery, R&D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, MA, USA., Summerfield JD; Encoded Library Technologies/NCE Molecular Discovery, R&D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, MA, USA. Jennifer.d.summerfield@gsk.com. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2541, pp. 207-214. |
| DOI: | 10.1007/978-1-0716-2545-3_24 |
| Abstrakt: | In this chapter, we describe a DEL "recipe" approach to hit confirmation using on-DNA cleavable linkers which can be followed by direct AS-MS evaluation and identification of binder(s) from the released small-molecule mixture. When coupled with automation, this platform creates opportunity for enabling an even higher-throughput binder confirmation (HTBC) platform to allow full interrogation of selections results. Two on-DNA cleavable strategies of releasing small molecules from DNA are reported herein. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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