Hsa_circ_0000479/Hsa-miR-149-5p/RIG-I, IL-6 Axis: A Potential Novel Pathway to Regulate Immune Response against COVID-19.

Autor: Firoozi Z; Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.; Department of Medical Genetics, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Mohammadisoleimani E; Department of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran., Shahi A; Department of Immunology, School of Medicine, Tehran University of Medical Science, Tehran, Iran.; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran., Naghizadeh MM; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran., Mirzaei E; Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran., Asad AG; Department of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran., Salmanpour Z; Department of Internal Medicine, Fasa University of Medical Sciences, Fasa, Iran., Javad Nouri SM; Department of Internal Medicine, Fasa University of Medical Sciences, Fasa, Iran., Mansoori Y; Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.
Jazyk: angličtina
Zdroj: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale [Can J Infect Dis Med Microbiol] 2022 Aug 30; Vol. 2022, pp. 2762582. Date of Electronic Publication: 2022 Aug 30 (Print Publication: 2022).
DOI: 10.1155/2022/2762582
Abstrakt: Background: COVID-19, the disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global pandemic and mortality of people around the world. Some circular RNAs (circRNAs), one of the new types of noncoding RNAs (ncRNAs), act as competing endogenous RNAs (ceRNAs) and compete with mRNAs for shared miRNAs, to regulate gene expression. In the present study, we aimed to evaluate the expression and roles of hsa_circ_0000479/hsa-miR-149-5p/RIG-I, IL-6 in COVID-19 infection.
Materials and Methods: After extraction of total RNA from peripheral blood mononuclear cells (PBMC) of 50 patients with symptomatic COVID-19, 50 patients with nonsymptomatic COVID-19, and 50 normal controls, cDNA synthesis was performed. Online in silico tools were applied to evaluate the interaction between the genes in the hsa_circ_0000479/hsa-miR-149-5p/RIG-I, IL-6 axis, and its role in COVID-19-related pathways. Quantification of the expression of these genes and confirmation of their interaction was done using the quantitative real-time PCR (qRT-PCR) technique.
Results: The expression levels of hsa_circ_0000479, RIG-I, and IL-6 were increased in COVID-19 patients compared to healthy controls, while hsa-miR-149-5p expression was decreased. Moreover, there was a significant negative correlation between hsa-miR-149-5p and hsa_circ_0000479, RIG-I, IL-6 expressions, and also a positive expression correlation between hsa_circ_0000479 and IL-6, RIG-I. Then, bioinformatics tools revealed the role of hsa_circ_0000479/hsa-miR-149-5p/RIG-I, IL-6 axis in PI3K-AKT and STAT3 signaling pathways.
Conclusion: Upregulation of hsa_circ_0000479, RIG-I, and IL-6, and downregulation of hsa-miR-149-5p, along with correlation studies, indicate that hsa_circ_0000479/hsa-miR-149-5p/RIG-I, IL-6 axis could play a role in regulating the immune response against SARS-CoV-2. However, more studies are needed in this area.
Competing Interests: All authors declare they have conflicts of interest.
(Copyright © 2022 Zahra Firoozi et al.)
Databáze: MEDLINE
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