| Autor: |
Terpilowska S; Jan Kochanowski University, Collegium Medicum, Department of Surgical Medicine with the Laboratory of Medical Genetics, IX Wieków Kielc 19A Av., 25-317 Kielce, Poland., Gluszek S; Jan Kochanowski University, Collegium Medicum, Department of Surgical Medicine with the Laboratory of Medical Genetics, IX Wieków Kielc 19A Av., 25-317 Kielce, Poland., Czerwosz E; Institute of Micro- and OptoElectronics, Warsaw Technical University, Nowowiejska 15/19, 00-665 Warszawa, Poland., Wronka H; Institute of Micro- and OptoElectronics, Warsaw Technical University, Nowowiejska 15/19, 00-665 Warszawa, Poland., Firek P; Institute of Micro- and OptoElectronics, Warsaw Technical University, Nowowiejska 15/19, 00-665 Warszawa, Poland., Szmidt J; Institute of Micro- and OptoElectronics, Warsaw Technical University, Nowowiejska 15/19, 00-665 Warszawa, Poland., Suchanska M; Kielce University of Technology, Al. Tysiąclecia Państwa Polskiego 7, 25-314 Kielce, Poland., Keczkowska J; Kielce University of Technology, Al. Tysiąclecia Państwa Polskiego 7, 25-314 Kielce, Poland., Kaczmarska B; Kielce University of Technology, Al. Tysiąclecia Państwa Polskiego 7, 25-314 Kielce, Poland., Kozlowski M; Łukasiewicz Research Network, Tele and Radio Research Institute, ul. Ratuszowa 11, 03-450 Warszawa, Poland., Diduszko R; Łukasiewicz Research Network, Institute of Microelectronics and Photonics, ul. Wólczyńskiej 133, 01-919 Warszawa, Poland. |
| Abstrakt: |
The application of nano-Ag grains as antiviral and antibacterial materials is widely known since ancient times. The problem is the toxicity of the bulk or big-size grain materials. It is known that nano-sized silver grains affect human and animal cells in some medical treatments. The aim of this study is to investigate the influence of nano-Ag grains embedded in a carbonaceous matrix on cytotoxicity, genotoxicity in fibroblasts, and mutagenicity. The nanocomposite film is composed of silver nanograins embedded in a carbonaceous matrix and it was obtained via the PVD method by deposition from two separated sources of fullerenes and silver acetate powders. This method allows for the preparation of material in the form of a film or powder, in which Ag nanograins are stabilized by a carbon network. The structure and morphology of this material were studied using SEM/EDX, XRD, and Raman spectroscopy. The toxicology studies were performed for various types of the material differing in the size of Ag nanograins. Furthermore, it was found that these properties, such as cell viability, genotoxicity, and mutagenicity, depend on Ag grain size. |
