Circ-TRIO promotes TNBC progression by regulating the miR-432-5p/CCDC58 axis.

Autor: Wang Z; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Li Y; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Yang J; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Liang Y; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Wang X; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Zhang N; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Kong X; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China., Chen B; Pathology Tissue Bank, Qilu Hospital of Shandong University, Jinan, China., Wang L; Pathology Tissue Bank, Qilu Hospital of Shandong University, Jinan, China., Zhao W; Pathology Tissue Bank, Qilu Hospital of Shandong University, Jinan, China., Yang Q; Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China. qifengy_sdu@163.com.; Pathology Tissue Bank, Qilu Hospital of Shandong University, Jinan, China. qifengy_sdu@163.com.; Research Institute of Breast Cancer, Shandong University, Jinan, China. qifengy_sdu@163.com.
Jazyk: angličtina
Zdroj: Cell death & disease [Cell Death Dis] 2022 Sep 08; Vol. 13 (9), pp. 776. Date of Electronic Publication: 2022 Sep 08.
DOI: 10.1038/s41419-022-05216-7
Abstrakt: Numerous studies have shown that circRNAs are aberrantly expressed in various cancers and play a significant role in tumor progression. However, the molecular mechanisms of circRNAs in triple-negative breast cancer (TNBC) remain ambiguous. By intersecting throughput data and qRT-PCR results from tissues and cell lines, circ-TRIO was identified as a potential oncogenic regulator of TNBC. Moreover, circ-TRIO expression was detected in TNBC tissues and was correlated with the recurrence and prognosis of TNBC patients. The circular characteristics of circ-TRIO were verified by RNase R and CHX assays. Functionally, the knockdown of circ-TRIO inhibited the proliferation, migration and invasion of TNBC cells, while the overexpression of circ-TRIO resulted in the opposite impacts. Mechanistically, a dual luciferase reporter assay and RNA immunoprecipitation were performed and indicated that circ-TRIO could combine with miR-432-5p to regulate the expression of coiled-coil domain containing 58 (CCDC58). In summary, our study illustrates that circ-TRIO plays an important role in the progression of TNBC by regulating the miR-432-5p/CCDC58 axis, which could broaden our insight into the underlying mechanisms and provide a novel prognostic marker of TNBC in the clinic.
(© 2022. The Author(s).)
Databáze: MEDLINE
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