The effect of dopaminergic neuron transplantation and melatonin co-administration on oxidative stress-induced cell death in Parkinson's disease.

Autor: Asemi-Rad A; Anatomy Department, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran., Moafi M; Anatomy and Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Aliaghaei A; Anatomy and Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Abbaszadeh HA; Anatomy and Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Abdollahifar MA; Anatomy and Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Ebrahimi MJ; Anatomy and Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Heidari MH; Anatomy and Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Sadeghi Y; Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Yousef.sadeghi@unimelb.edu.au.; Department of Anatomy and Neuroscience, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Melbourne, VlC, Australia. Yousef.sadeghi@unimelb.edu.au.
Jazyk: angličtina
Zdroj: Metabolic brain disease [Metab Brain Dis] 2022 Dec; Vol. 37 (8), pp. 2677-2685. Date of Electronic Publication: 2022 Sep 08.
DOI: 10.1007/s11011-022-01021-5
Abstrakt: A gradual degeneration of the striatum and loss of nigral dopamine cells are characteristic of Parkinson's disease. Nowadays, combination therapy for neurodegenerative disease is considered. This study aimed to investigate the effects of melatonin and dopaminergic neurons derived from adipose tissue stem cells (ADSCs) in a rat model of Parkinson's disease. Parkinson's disease was induced in rats using neurotoxin 6-Hydroxydopamine. The treatment was performed using melatonin and dopaminergic neurons transplantation. Subsequently, behavioral tests, western blot analysis for Caspase-3 expression, GSH (Glutathione) content and stereology analysis for the volume and cell number of substantia nigra and striatum were performed. Treatment with melatonin and dopaminergic neuron transplantation increased the number of neurons in substantia nigra and striatum while the number of glial cell and the volume of substantia nigra and striatum did not show significant change between groups. Western blot analysis for caspase 3 indicated the significant differences between groups. The results also indicated the increased level of glutathione (GSH) content in treatment groups. this study showed that combination therapy with melatonin and dopaminergic neurons could greatly protect the neurons, reduce oxidative stress and improve the symptoms of PD.
(© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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