De novo assembly and annotation of the CHOZN® GS -/- genome supports high-throughput genome-scale screening.

Autor: Kretzmer C; Upstream Research and Development, MilliporeSigma, St. Louis, Missouri, USA., Narasimhan RL; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Lal RD; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Balassi V; Upstream Research and Development, MilliporeSigma, St. Louis, Missouri, USA., Ravellette J; Upstream Research and Development, MilliporeSigma, St. Louis, Missouri, USA., Kotekar Manjunath AK; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Koshy JJ; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Viano M; Istituto di Ricerche Biomediche 'A. Marxer' RBM S.p.A., Ivrea, Italy., Torre S; Istituto di Ricerche Biomediche 'A. Marxer' RBM S.p.A., Ivrea, Italy., Zanda VM; Istituto di Ricerche Biomediche 'A. Marxer' RBM S.p.A., Ivrea, Italy., Kumravat M; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Saldanha KMR; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Chandranpillai H; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Nihad I; Bioinformatics, IT R&D Applications, Merck (Sigma-Aldrich Chemicals Pvt. Ltd., A subsidiary of Merck KGaA, Darmstadt, Germany), Bangalore, India., Zhong F; Life Science Bioinformatics, IT, MilliporeSigma, St. Louis, Missouri, USA., Sun Y; Bioinformatics, IT R&D Applications, MilliporeSigma, St. Louis, Missouri, USA., Gustin J; Upstream Research and Development, MilliporeSigma, St. Louis, Missouri, USA., Borgschulte T; Upstream Research and Development, MilliporeSigma, St. Louis, Missouri, USA., Liu J; Life Science Bioinformatics, IT, MilliporeSigma, St. Louis, Missouri, USA., Razafsky D; Upstream Research and Development, MilliporeSigma, St. Louis, Missouri, USA.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2022 Dec; Vol. 119 (12), pp. 3632-3646. Date of Electronic Publication: 2022 Sep 23.
DOI: 10.1002/bit.28226
Abstrakt: Chinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial-scale production processes and media, the CHO cell has remained largely unchanged. Due to the cost and complexity of whole-genome sequencing and gene-editing it has been difficult to obtain the tools necessary to improve the CHO cell line. With the advent of next-generation sequencing and the discovery of the CRISPR/Cas9 system it has become more cost effective to sequence and manipulate the CHO genome. Here, we provide a comprehensive de novo assembly and annotation of the CHO-K1 based CHOZN® GS -/- genome. Using this platform, we designed, built, and confirmed the functionality of a whole genome CRISPR guide RNA library that will allow the bioprocessing community to design a more robust CHO cell line leading to the production of life saving medications in a more cost-effective manner.
(© 2022 Sigma Aldrich Co LLC. Biotechnology and Bioengineering published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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