Between-day reliability of cytokines and adipokines for application in research and practice.

Autor:	Rose GL; School of Nursing, Midwifery and Social Work, The University of Queensland, Brisbane, QLD, Australia.; School of Human Movement and Nutrition Sciences, The University of Queensland, Brisbane, QLD, Australia.; The School of Health and Behavioural Sciences, University of the Sunshine Coast, Sippy Downs, QLD, Australia., Farley MJ; School of Human Movement and Nutrition Sciences, The University of Queensland, Brisbane, QLD, Australia., Flemming NB; School of Medicine and Dentistry, Griffith University, Birtinya, QLD, Australia.; The School of Health and Behavioural Sciences, University of the Sunshine Coast, Sippy Downs, QLD, Australia.; Sunshine Coast Health Institute, Birtinya, QLD, Australia., Skinner TL; School of Human Movement and Nutrition Sciences, The University of Queensland, Brisbane, QLD, Australia., Schaumberg MA; School of Human Movement and Nutrition Sciences, The University of Queensland, Brisbane, QLD, Australia.; The School of Health and Behavioural Sciences, University of the Sunshine Coast, Sippy Downs, QLD, Australia.; Sunshine Coast Health Institute, Birtinya, QLD, Australia.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2022 Aug 22; Vol. 13, pp. 967169. Date of Electronic Publication: 2022 Aug 22 (Print Publication: 2022).
DOI:	10.3389/fphys.2022.967169
Abstrakt:	Purpose: This study assessed the biological reliability of peripheral human cytokines and adipokines, and the influence of participant characteristics on total error. This has essential application to interventional cytokine measurement to ensure that reported results are interpreted with confidence. Methods: Participants (49% female, 18-85 years, n = 84) completed two consecutive-day testing sessions. Participants provided a venous blood sample at the same time of day across two consecutive days, under standardized participant presentation, including 24-h rested and 12-h fasted conditions. Multiplex immunoassay was used to assess inflammatory analytes from samples (predominantly plasma). Repeat measurements were conducted between-day for total precision quantification, and technical (technique) error was negated from the total to provide an estimate of biological (attributed to participant presentation) error. Results: Whilst there was no evidence of statistically significant biological error, a small amount of biological error was consistently present across most analytes (∼3.3%/0.07 pg/ml), which was largest for measurement of leptin (7.3%/210 pg/ml). There was also an influence of sex on reliability of leptin and adiponectin (total model explained 6-7% of error variation), where females demonstrated the greatest error. Conclusion: Biological error reported in this study should be applied to any future study or individual with a repeated measurement of cytokine concentrations over time that maintain best practice procedures (12-h fasted, 24-h rested). In most cases, raw error should be used, with exceptions for women for measurement of leptin and adiponectin. This approach will ensure that results are reported with certainty for improved reporting of intervention efficacy. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Rose, Farley, Flemming, Skinner and Schaumberg.)
Databáze:	MEDLINE
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