Development of a murine model of oral carcinogenesis: an accelerated tool for biomarker and anti-tumour drug discovery.

Autor:	Syed SA; Department of Oral Pathology, Dr. Ishrat-ul-Ibad Khan Institute of Oral Health Sciences, Dow University of Health Sciences, Karachi 74200, Pakistan., Qureshi MA; Department of Pathology, Dow International Medical College, Dow University of Health Sciences, Karachi 74200, Pakistan.; https://orcid.org/0000-0002-8370-1069., Khan S; Department of Pathology, Dow International Medical College, Dow University of Health Sciences, Karachi 74200, Pakistan., Kumar R; Department of ENT, Dr. Ruth K.M. Pfau, Civil Hospital, Dow University of Health Sciences, Karachi 74200, Pakistan., Shafique Y; Department of Pathology, Dow Medical College, Dow University of Health Sciences, Karachi 74200, Pakistan., Khan BA; Department of Pathology, Dow International Medical College, Dow University of Health Sciences, Karachi 74200, Pakistan., Safdar J; Department of Oral & Maxillofacial Surgery, Dow Dental College, Dow University of Health Sciences, Karachi 74200, Pakistan.
Jazyk:	angličtina
Zdroj:	Ecancermedicalscience [Ecancermedicalscience] 2022 Jun 15; Vol. 16, pp. 1413. Date of Electronic Publication: 2022 Jun 15 (Print Publication: 2022).
DOI:	10.3332/ecancer.2022.1413
Abstrakt:	Oral squamous cell carcinoma (OSCC) is the most common cancer in Pakistani men and the second most common cancer in women. The objective of our study was to devise a novel accelerated murine model of oral carcinogenesis that can be exploited as a tool to investigate the cancer circuitry involved in OSCC and to identify molecules of diagnostic, therapeutic and prognostic significance. A total of 40 healthy male, 6-8 weeks old, 22 ± 2 gram, Naval Medical Research Institute (NMRI) outbred strain mice were recruited in the experiment. NMRI mice are commonly used for animal experiments in various fields of biology and for drug toxicity. Of these, 25 mice underwent the oral carcinogenesis regimen via topical application of 0.5% 9,10-dimethyl-1,2-benzanthracene (DMBA) on the lower left lip for a maximum of 20 weeks and 15 mice were used as controls (without the carcinogenic regimen). Exophytic tissue masses were harvested, fixed in 10% formalin and stained with haematoxylin and eosin (H&E) for microscopic diagnosis. Additionally, the expression levels of CK 5/6, p53 and Ki-67 were investigated using immunohistochemistry. Of the 25 mice which underwent the carcinogenic regimen, 21 developed moderately differentiated squamous cell carcinoma and 1 showed dysplastic features with foci of invasion. Three mice were found dead with lesion(s). CK 5/6 showed strong positivity (100%) and p53 and Ki-67 showed patchy (<30%) strong positivity in OSCC, suggesting the similarity of our model to human OSCC. We present an accelerated, close-to-human carcinogenesis, model of oral carcinogenesis using DMBA in NMRI mice that can be exploited to study the pathogenesis of oral squamous cell carcinoma and subsequently devise immunotherapy or targeted therapy. Competing Interests: The authors declare that they have no conflicts of interest. (© the authors; licensee ecancermedicalscience.)
Databáze:	MEDLINE
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