Backbone and side chain NMR assignments for the ribosome maturation factor P (RimP) from Staphylococcus aureus.

Autor: Garaeva NS; Kazan Federal University, 18 Kremlevskaya, Kazan, Russian Federation, 420008.; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008., Bikmullin AG; Kazan Federal University, 18 Kremlevskaya, Kazan, Russian Federation, 420008.; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008., Fatkhullin BF; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008.; Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, Université de Strasbourg, 1 rue Laurent Fries, 67400, Illkirch, France.; Institute of Protein Research, Russian Academy of Science, Institutskaya 4, Pushchino, Moscow Region, Russian Federation, 142290., Validov SZ; Kazan Federal University, 18 Kremlevskaya, Kazan, Russian Federation, 420008.; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008., Keiffer B; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008.; Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, Université de Strasbourg, 1 rue Laurent Fries, 67400, Illkirch, France., Yusupov MM; Kazan Federal University, 18 Kremlevskaya, Kazan, Russian Federation, 420008.; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008.; Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, Université de Strasbourg, 1 rue Laurent Fries, 67400, Illkirch, France., Usachev KS; Kazan Federal University, 18 Kremlevskaya, Kazan, Russian Federation, 420008. k.usachev@kpfu.ru.; Federal Research Center, «Kazan Scientific Center of Russian Academy of Sciences», Russia, Lobachevskogo, 2/31, Kazan, Russian Federation, 420008. k.usachev@kpfu.ru.
Jazyk: angličtina
Zdroj: Biomolecular NMR assignments [Biomol NMR Assign] 2022 Oct; Vol. 16 (2), pp. 373-377. Date of Electronic Publication: 2022 Sep 07.
DOI: 10.1007/s12104-022-10106-2
Abstrakt: The ribosomal maturation factor (RimP) is a 17.7 kDa protein and is the assembly factor of the 30S subunit. RimP is essential for efficient processing of 16S rRNA and maturation (assembly) of the 30S ribosome. It was suggested that RimP takes part in stabilization of the central pseudoknot at the early stages of the 30S subunit maturation, and this process may occur before the head domain assembly and later stages of the 30S assembly, but the mechanism of this interaction is still not fully understood. Here we report the assignment of the 1 H, 13 C and 15 N chemical shift in the backbone and side chains of RimP from Staphylococcus aureus. Analysis of chemical shifts of the main chain using TALOS + suggests that the RimP contains eight β-strands and three α-helices with the topology α1-β1-β2-α2- β3- α3- β4- β5- β6- β7- β8. Structural studies of RimP and its complex with the ribosome by integrated structural biology approaches (NMR spectroscopy, X-ray diffraction analysis and cryoelectron microscopy) will allow further screening of highly selective inhibitors of the translation of S. aureus.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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