Full and Partial Mid-substance ACL Rupture Using Mechanical Tibial Displacement in Male and Female Mice.

Autor: Timkovich AE; Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO, 80523-1621, USA., Sikes KJ; Department of Clinical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Andrie KM; Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO, 80523-1621, USA., Afzali MF; Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO, 80523-1621, USA., Sanford J; Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO, 80523-1621, USA., Fernandez K; Department of Clinical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Burnett DJ; Department of Clinical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Hurley E; Department of Clinical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Daniel T; Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO, 80523-1621, USA., Serkova NJ; Department of Radiology, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA., Donahue TH; University of Memphis, Memphis, TN, 38152, USA., Santangelo KS; Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO, 80523-1621, USA. Kelly.Santangelo@colostate.edu.
Jazyk: angličtina
Zdroj: Annals of biomedical engineering [Ann Biomed Eng] 2023 Mar; Vol. 51 (3), pp. 579-593. Date of Electronic Publication: 2022 Sep 07.
DOI: 10.1007/s10439-022-03065-1
Abstrakt: The anterior cruciate ligament (ACL) is the most commonly injured knee ligament. Surgical reconstruction is the gold standard treatment for ACL ruptures, but 20-50% of patients develop post-traumatic osteoarthritis (PTOA). ACL rupture is thus a well-recognized etiology of PTOA; however, little is known about the initial relationship between ligamentous injury and subsequent PTOA. The goals of this project were to: (1) develop both partial and full models of mid-substance ACL rupture in male and female mice using non-invasive mechanical methods by means of tibial displacement; and (2) to characterize early PTOA changes in the full ACL rupture model. A custom material testing system was utilized to induce either partial or full ACL rupture by means of tibial displacement at 1.6 or 2.0 mm, respectively. Mice were euthanized either (i) immediately post-injury to determine rupture success rates or (ii) 14 days post-injury to evaluate early PTOA progression following full ACL rupture. Our models demonstrated high efficacy in inciting either full or partial ACL rupture in male and female mice within the mid-substance of the ACL. These tools can be utilized for preclinical testing of potential therapeutics and to further our understanding of PTOA following ACL rupture.
(© 2022. The Author(s) under exclusive licence to Biomedical Engineering Society.)
Databáze: MEDLINE
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