Lipidome Analysis in Brain and Peripheral Plasma Following Milk Fat Globule Membrane Supplementation in Rodents.

Autor: Davies R; Medical and Scientific Affairs, Reckitt|Mead Johnson Nutrition Institute, Slough, SL1 4AA, UK., van Diepen JA; Medical and Scientific Affairs, Reckitt|Mead Johnson Nutrition Institute, Nijmegen, 6545CJ, The Netherlands., Brink LR; Medical and Scientific Affairs, Reckitt|Mead Johnson Nutrition Institute, Evansville, WI, 47712, USA., Bijlsma S; Netherlands Organization for Applied Scientific Research (TNO), Utrecht/Leiden, 2333CK, The Netherlands., Neufeld KM; APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland., Cryan JF; APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland.; Department of Anatomy and Neuroscience, University College Cork, Cork, T12 K8AF, Ireland., O'Mahony SM; APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland.; Department of Anatomy and Neuroscience, University College Cork, Cork, T12 K8AF, Ireland., Bobeldijk I; Netherlands Organization for Applied Scientific Research (TNO), Utrecht/Leiden, 2333CK, The Netherlands., Gross G; Medical and Scientific Affairs, Reckitt|Mead Johnson Nutrition Institute, Nijmegen, 6545CJ, The Netherlands.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2022 Nov; Vol. 66 (22), pp. e2200177. Date of Electronic Publication: 2022 Oct 07.
DOI: 10.1002/mnfr.202200177
Abstrakt: Scope: Milk fat globule membrane (MFGM) is an essential component of milk. Bovine MFGM (bMFGM) has been shown to support cognitive development and increase relative concentrations of serum phospholipids. This study investigates bioavailability of bMFGM components after oral administration in two preclinical models to explore whether dietary bMFGM induces parallel changes to plasma and brain lipidomes.
Methods and Results: Transgenic APOE*3.Leiden mice (n = 18 per group) and Sprague-Dawley rats (n = 12 per group) are fed bMFGM-enriched (MFGM+) or Control diet, followed by phospholipid profile-determination in plasma, hippocampus, and prefrontal cortex tissue by targeted mass spectrometry. Multivariate analysis of lipidomic profiles demonstrates a separation between MFGM+ and Control plasma across rodents. In plasma, sphingomyelins contributed the most to the separation of lipid patterns among both models, where three sphingomyelins (d18:1/14:0, d18:1/23:0, d18:1/23:1[9Z]) are consistently higher in the circulation of MFGM+ groups. A similar trend is observed in rat prefrontal cortex, although no significant separation of the brain lipidome is demonstrated.
Conclusion: bMFGM-enriched diet alters plasma phospholipid composition in rodents, predominantly increasing sphingomyelin levels in the systemic circulation with similar, but non-significant, trends in central brain regions. These changes may contribute to the beneficial effects of bMFGM on neurodevelopment during early life.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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