TiO 2 nanoparticles added to dense bovine hydroxyapatite bioceramics increase human osteoblast mineralization activity.

Autor: Fernandes PHM; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Bordini EAF; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Cassiano FB; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., de Azevedo-Silva LJ; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Ferrairo BM; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Lisboa-Filho PN; Department of Physics, School of Sciences, São Paulo State University, Bauru, SP, Brazil., Fortulan CA; Department of Mechanical Engineering, São Carlos School of Engineering, University of São Paulo, São Carlos, SP, Brazil., Soares Dos Passos DG; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Borges AFS; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: afborges@fob.usp.br.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2022 Nov; Vol. 38 (11), pp. e275-e283. Date of Electronic Publication: 2022 Sep 06.
DOI: 10.1016/j.dental.2022.08.007
Abstrakt: Objectives: This study evaluated the effect of TiO 2 nanoparticles + dense hydroxyapatite (HA) on human osteoblast cells (SAOS-2).
Methods: Particulate bovine HA powder with or without the addition of either 5 or 8 % TiO 2 (HA, HA/TiO 2 Np5 % or HA/TiO 2 Np8 %) were pressed into disks (Ø = 12.5 mm; thickness = 1.3 mm) uniaxially (100 MPa) and isostatically (200 MPa/1 min) and sintered at 1300 °C. Y-TZP disks were used as control. The following tests were performed: Scanning Electron Microscopy and Dispersive Energy Spectroscopy (SEM/EDS), Atomic Force Microscopy (AFM), cell viability assay (Alamar Blue-AB) and mineralized matrix deposition (Alizarin Red-AR). AB and AR data were submitted to 2-way ANOVA/Tukey tests and ANOVA/Tukey tests, respectively.
Results: SEM revealed that the surface of HA/TiO 2 Np5% resembles DPBHA surface, but also contains smaller granules. HA/TiO 2 Np8% characteristics resembles HA/TiO 2 Np5% surface, but with irregular topography. Y-TZP showed a typical oxide ceramic surface pattern. EDS revealed Ca, O, and P in all samples. C, O, and Zr appeared in Y-TZP samples. AFM data corroborates SEM analysis. AB test revealed excellent cellular viability for HA/TiO 2 Np5% group. AR test showed that all groups containing TiO 2 np had more mineralized matrix deposition than all other groups, with statistically differences between HA/TiO 2 Np8% and HA cultivated in non-osteogenic medium. Culture in osteogenic medium exhibited much more mineralized matrix deposition by TiO 2 np groups.
Significance: In conclusion, the addition of TiO 2 np showed chemical, superficial, and biological changes in the reinforced materials. HA/TiO 2 Np5% showed the best results for cell viability and HA/TiO 2 Np8% for mineralized matrix deposition.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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