1α,25-Dihydroxyvitamin D3 (VD3) Shows a Neuroprotective Action Against Rotenone Toxicity on PC12 Cells: An In Vitro Model of Parkinson's Disease.

Autor: de Siqueira EA; Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Magalhães EP; Department of Clinical and Toxicological Analysis, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., de Assis ALC; Department of Clinical Medicine, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Sampaio TL; Department of Clinical and Toxicological Analysis, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Lima DB; Department of Clinical and Toxicological Analysis, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Marinho MM; Department of Clinical and Toxicological Analysis, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Martins AMC; Department of Clinical and Toxicological Analysis, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., de Andrade GM; Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., de Barros Viana GS; Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil. gbviana@live.com.
Jazyk: angličtina
Zdroj: Neurochemical research [Neurochem Res] 2023 Jan; Vol. 48 (1), pp. 250-262. Date of Electronic Publication: 2022 Sep 06.
DOI: 10.1007/s11064-022-03735-5
Abstrakt: Parkinson's disease (PD) is characterized by dopaminergic cell loss in the substantia nigra, and PD brains show neuroinflammation, oxidative stress, and mitochondrial dysfunction. The study evaluated the neuroprotective activity of 1α,25-dihydroxy vitamin D3 (VD3), on the rotenone (ROT)-induced cytotoxicity in PC12 cells. The viability parameters were assessed by the MTT and flow cytometry, on cells treated or not with VD3 and/or ROT. Besides, ROS production, cell death, mitochondrial transmembrane potential, reduced GSH, superoxide accumulation, molecular docking (TH and Keap1-Nrf2), and TH, Nrf2, NF-kB, and VD3 receptor protein contents by western blot were evaluated. VD3 was shown to improve the viability of ROT-exposed cells. Cells exposed to ROT showed increased production of ROS and superoxide, which decreased after VD3. ROT decrease in the mitochondrial transmembrane potential was prevented, after VD3 treatment and, VD3 was shown to interact with tyrosine hydroxylase (TH) and Nrf2. While ROT decreased TH, Nrf2, and NF-kB expressions, these effects were reversed by VD3. In addition, VD3 also increased VD3 receptor protein contents and values went back to those of controls after ROT exposure. VD3 protects PC12 cells against ROT damage, by decreasing oxidative stress and improving mitochondrial function. One target seems to be the TH molecule and possibly an indirect Nrf2 activation could also justify its neuroprotective actions on this PC12 cell model of PD.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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