Reproducible propagation technique for the symbiotic cnidarian model system Cassiopea xamachana.

Autor:	Newkirk C; Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA., Vadlapudi S; Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA., Sadula M; Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA., Arbello C; Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA., Xiang T; Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Jazyk:	angličtina
Zdroj:	Biology open [Biol Open] 2022 Sep 15; Vol. 11 (9). Date of Electronic Publication: 2022 Sep 13.
DOI:	10.1242/bio.059413
Abstrakt:	The phylum Cnidaria is composed of corals, jellyfish, hydras, and sea anemones. Cnidarians are well-known for their regenerative capability, with many species maintaining the ability to regenerate complete structures. This regenerative capacity has been used casually for propagation purposes (via dissection) for some cnidarians used in laboratory research but has yet been documented in a manner meant to be reproducible. One such cnidarian model system is the scyphozoan jellyfish Cassiopea xamachana. C. xamachana has become an emerging model system for studying the cnidarian-algal symbiotic relationship, so determining a reliable and fast method for expansion of laboratory animals is crucial. Here we outline a reproducible propagation method for continued generation and growth of C. xamachana polyps. This article has an associated First Person interview with the first author of the paper. Competing Interests: Competing interests The authors declare that the research performed in this protocol was conducted in the absence of any competing or conflicting interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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