Green method for improving performance attributes of wool fibres using immobilized proteolytic thermozyme.

Autor: Gomaa SK; Chemistry of Natural and Microbial Products Department, National Research Centre, Giza, 12622 Dokki Egypt., Zaki RA; Chemistry of Natural and Microbial Products Department, National Research Centre, Giza, 12622 Dokki Egypt., Wahba MI; Chemistry of Natural and Microbial Products Department, National Research Centre, Giza, 12622 Dokki Egypt.; Centre of Scientific Excellence-Group of Advanced Materials and Nanotechnology, National Research Centre, Giza, 12622 Dokki Egypt., Taleb MA; Proteinic and Man-made Fibres Department, Textile Research and Technology Institute, National Research Centre, Giza, 12622 Dokki Egypt., El-Refai HA; Chemistry of Natural and Microbial Products Department, National Research Centre, Giza, 12622 Dokki Egypt., El-Fiky AF; Proteinic and Man-made Fibres Department, Textile Research and Technology Institute, National Research Centre, Giza, 12622 Dokki Egypt., El-Sayed H; Proteinic and Man-made Fibres Department, Textile Research and Technology Institute, National Research Centre, Giza, 12622 Dokki Egypt.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2022 Oct; Vol. 12 (10), pp. 254. Date of Electronic Publication: 2022 Sep 02.
DOI: 10.1007/s13205-022-03323-y
Abstrakt: Wool has the tendency to turn into felt during agitation in washing machines. Thus, a benign non-polluting method for the production of machine-washable wool was developed herein. Initially, a proteolytic bacteria was isolated from hot region soil. The bacterial isolate was identified as  Bacillus safensis FO-36bMZ836779 according to the 16S rRNA gene sequencing. Afterwards, the extracellular protease produced by this isolate was covalently immobilized in order to enhance its stability under non-ambient conditions which are usually adopted in industrial sectors like textile industries. Sericin, which is usually discharged into degumming effluent of natural silk, was utilized to prepare the immobilization carrier. Box-Behnken design was adopted in order to hone the preparation of the sericin-polyethylene-imine-glutaraldehyde activated agar carrier. The pH and temperature profiles of the free and immobilized proteases were compared. Later, wool fibres were bio-treated with both the free and the immobilized enzymes. The effect of process conditions on the resistance of the bio-finished wool to felting was investigated. The alteration in the fibre morphology was monitored using SEM. Amino acid analysis and alkali solubility tests were adopted to assign any change in the chemical structure of the bio-treated wool. The influence of bio-treatment of wool on its inherent properties was assigned. Results revealed that bio-treatment of wool with the said enzyme led to production of machine-washable wool without severe deterioration in the fibres' properties. In an energy- and water-consuming process, the hot solution from bio-treatment bath was used successfully in dyeing of wool.
Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03323-y.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest.
(© The Author(s) 2022.)
Databáze: MEDLINE
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