Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus .

Autor:	Pomraning KR; Pacific Northwest National Laboratory, Richland, WA, USA., Dai Z; Pacific Northwest National Laboratory, Richland, WA, USA., Munoz N; Pacific Northwest National Laboratory, Richland, WA, USA., Kim YM; Pacific Northwest National Laboratory, Richland, WA, USA., Gao Y; Pacific Northwest National Laboratory, Richland, WA, USA., Deng S; Pacific Northwest National Laboratory, Richland, WA, USA., Lemmon T; Pacific Northwest National Laboratory, Richland, WA, USA., Swita MS; Pacific Northwest National Laboratory, Richland, WA, USA., Zucker JD; Pacific Northwest National Laboratory, Richland, WA, USA., Kim J; Pacific Northwest National Laboratory, Richland, WA, USA., Mondo SJ; Joint Genome Institute, Walnut Creek, CA, USA., Panisko E; Pacific Northwest National Laboratory, Richland, WA, USA., Burnet MC; Pacific Northwest National Laboratory, Richland, WA, USA., Webb-Robertson BM; Pacific Northwest National Laboratory, Richland, WA, USA., Hofstad B; Pacific Northwest National Laboratory, Richland, WA, USA., Baker SE; Pacific Northwest National Laboratory, Richland, WA, USA.; Joint BioEnergy Institute, Emeryville, CA, USA., Burnum-Johnson KE; Pacific Northwest National Laboratory, Richland, WA, USA., Magnuson JK; Pacific Northwest National Laboratory, Richland, WA, USA.; Joint BioEnergy Institute, Emeryville, CA, USA.
Jazyk:	angličtina
Zdroj:	Metabolic engineering communications [Metab Eng Commun] 2022 Aug 24; Vol. 15, pp. e00203. Date of Electronic Publication: 2022 Aug 24 (Print Publication: 2022).
DOI:	10.1016/j.mec.2022.e00203
Abstrakt:	The global regulator LaeA controls secondary metabolism in diverse Aspergillus species. Here we explored its role in regulation of itaconic acid production in Aspergillus pseudoterreus . To understand its role in regulating metabolism, we deleted and overexpressed laeA, and assessed the transcriptome, proteome, and secreted metabolome prior to and during initiation of phosphate limitation induced itaconic acid production. We found that secondary metabolite clusters, including the itaconic acid biosynthetic gene cluster, are regulated by laeA and that laeA is required for high yield production of itaconic acid. Overexpression of LaeA improves itaconic acid yield at the expense of biomass by increasing the expression of key biosynthetic pathway enzymes and attenuating the expression of genes involved in phosphate acquisition and scavenging. Increased yield was observed in optimized conditions as well as conditions containing excess nutrients that may be present in inexpensive sugar containing feedstocks such as excess phosphate or complex nutrient sources. This suggests that global regulators of metabolism may be useful targets for engineering metabolic flux that is robust to environmental heterogeneity. Competing Interests: The authors declare no competing interests. (© 2022 The Authors.)
Databáze:	MEDLINE
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