Stem Cell Exosomes Improve Survival of Neural Stem Cells after Radiation Exposure.

Autor: Ratushnyak MG; National Research Center 'Kurchatov Institute', Moscow, Russia. ratushnyak_marya@mail.ru., Semochkina YP; National Research Center 'Kurchatov Institute', Moscow, Russia., Yastremsky EV; National Research Center 'Kurchatov Institute', Moscow, Russia., Kamyshinsky RA; National Research Center 'Kurchatov Institute', Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Aug; Vol. 173 (4), pp. 544-552. Date of Electronic Publication: 2022 Sep 05.
DOI: 10.1007/s10517-022-05587-z
Abstrakt: The death of neural stem cells in the hippocampus during radiation therapy of brain tumors leads to neurogenesis impairment and the development of cognitive dysfunctions at delayed terms after irradiation. Exosomes secreted by stem cells can provide a protective effect on neural stem cells. We isolated and characterized exosomes from the medium conditioned by neural stem cells and mesenchymal stem cells from mouse adipose tissue and studied their efficiency in protecting irradiated neural stem cells. According to dynamic light scattering data, the exosome size varied from 44 to 68 nm for neural stem cells, and from 80 to 130 nm for mesenchymal stem cells. All exosomes carried markers CD9, CD63, and TSG101. The survival rate and clonogenic activity of neural stem cells irradiated in a dose of 1 Gy was found to increase after culturing in the presence of stem cell exosome preparations.
(© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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