Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep.

Autor:	Hitit M; Department of Genetics, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey., Kose M; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey., Kaya MS; Department of Physiology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey., Kırbas M; Bahri Dagdas International Agricultural Research Institute, Konya, Turkey., Dursun S; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkey., Alak I; Department of Animal Sciences, Vocational School of Technical Sciences, Ankara Yıldırım Beyazıt University, Ankara, Turkey., Atli MO; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey.
Jazyk:	angličtina
Zdroj:	Frontiers in genetics [Front Genet] 2022 Aug 17; Vol. 13, pp. 929477. Date of Electronic Publication: 2022 Aug 17 (Print Publication: 2022).
DOI:	10.3389/fgene.2022.929477
Abstrakt:	MicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Hitit, Kose, Kaya, Kırbas, Dursun, Alak and Atli.)
Databáze:	MEDLINE
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