Vibration therapy as an effective approach to improve bone healing in diabetic rats.

Autor:	Campos MS; Department of Orthopaedics and Anaesthesiology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil., Volpon JB; Department of Orthopaedics and Anaesthesiology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil., Ximenez JPB; Laboratory of Molecular Biology, Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, Ribeirão Preto, SP, Brazil.; School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo, Ribeirão Preto, SP, Brazil., Franttini AP; Department of Orthopaedics and Anaesthesiology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil., Dalloul CE; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Sousa-Neto MD; School of Dentistry of Ribeirão Preto - University of São Paulo, Ribeirão Preto, SP, Brazil., Silva RA; School of Dentistry of Ribeirão Preto - University of São Paulo, Ribeirão Preto, SP, Brazil., Kacena MA; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States.; Richard L. Roudebush Veterans Affairs Medical Center, Indianapolis, IN, United States., Zamarioli A; Department of Orthopaedics and Anaesthesiology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Aug 19; Vol. 13, pp. 909317. Date of Electronic Publication: 2022 Aug 19 (Print Publication: 2022).
DOI:	10.3389/fendo.2022.909317
Abstrakt:	Objective: To investigate the effects of vibration therapy on fracture healing in diabetic and non-diabetic rats. Methods: 148 rats underwent fracture surgery and were assigned to four groups: (1) SHAM: weight-matched non-diabetic rats, (2) SHAM+VT: non-diabetic rats treated with vibration therapy (VT), (3) DM: diabetic rats, and (4) DM+VT: diabetic rats treated with VT. Thirty days after diabetes induction with streptozotocin, animals underwent bone fracture, followed by surgical stabilization. Three days after bone fracture, rats began VT. Bone healing was assessed on days 14 and 28 post-fracture by serum bone marker analysis, and femurs collected for dual-energy X-ray absorptiometry, micro-computed tomography, histology, and gene expression. Results: Our results are based on 88 animals. Diabetes led to a dramatic impairment of bone healing as demonstrated by a 17% reduction in bone mineral density and decreases in formation-related microstructural parameters compared to non-diabetic control rats (81% reduction in bone callus volume, 69% reduction in woven bone fraction, 39% reduction in trabecular thickness, and 45% in trabecular number). These changes were accompanied by a significant decrease in the expression of osteoblast-related genes ( Runx2, Col1a1, Osx ), as well as a 92% reduction in serum insulin-like growth factor I (IGF-1) levels. On the other hand, resorption-related parameters were increased in diabetic rats, including a 20% increase in the callus porosity, a 33% increase in trabecular separation, and a 318% increase in serum C terminal telopeptide of type 1 collagen levels. VT augmented osteogenic and chondrogenic cell proliferation at the fracture callus in diabetic rats; increased circulating IGF-1 by 668%, callus volume by 52%, callus bone mineral content by 90%, and callus area by 72%; and was associated with a 19% reduction in circulating receptor activator of nuclear factor kappa beta ligand (RANK-L). Conclusions: Diabetes had detrimental effects on bone healing. Vibration therapy was effective at counteracting the significant disruption in bone repair induced by diabetes, but did not improve fracture healing in non-diabetic control rats. The mechanical stimulus not only improved bone callus quality and quantity, but also partially restored the serum levels of IGF-1 and RANK-L, inducing bone formation and mineralization, thus creating conditions for adequate fracture repair in diabetic rats. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Campos, Volpon, Ximenez, Franttini, Dalloul, Sousa-Neto, Silva, Kacena and Zamarioli.)
Databáze:	MEDLINE
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