Relationship between serum and tear levels of tissue plasminogen activator and plasminogen activator inhibitor-1 in diabetic retinopathy.

Autor: Jasmiad NB; Department of Internal Medicine, Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor Darul Ehsan, Malaysia. keey_85@yahoo.com., Abd Ghani RB; Department of Internal Medicine, Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor Darul Ehsan, Malaysia. agrohana@gmail.com.; Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor Darul Ehsan, Malaysia. agrohana@gmail.com., Agarwal R; Department of Pharmacology and Therapeutics, School of Medicine, International Medical University, Kuala Lumpur, Malaysia. renuagarwal@imu.edu.my., Ismail ZB; Department of Public Health Medicine, Faculty of Medicine, Universiti Teknology MARA (UiTM), Sungai Buloh, Selangor Darul Ehsan, Malaysia., Mohd Abdullah AA; MSU Medical Centre, Persiaran Olahraga, Shah Alam, Selangor Darul Ehsan, Malaysia.; MSU Clinical Centre of Excellence, Management and Science University, Shah Alam, Selangor Darul Ehsan, Malaysia., Idorus MY; Institute of Medical Molecular Biotechnology (IMMB), Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor Darul Ehsan, Malaysia.
Jazyk: angličtina
Zdroj: BMC ophthalmology [BMC Ophthalmol] 2022 Sep 03; Vol. 22 (1), pp. 357. Date of Electronic Publication: 2022 Sep 03.
DOI: 10.1186/s12886-022-02550-4
Abstrakt: Background: Diabetic retinopathy (DR) is a serious complication of longstanding type 2 diabetes mellitus (T2DM), a leading cause of blindness and visual disability in the world. The aim of this study is to compare the activity of tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) in tears and serum of patients with DR and those without DR.
Method: Among the T2DM patients enrolled in this study, 26 patients had DR (n = 26) while 29 were without DR (n = 29). The blood and tear samples were obtained from all participants. The level of PAI-1 and tPA were measured in both the serum and tears. Anthropometric measurements, HbA1c, renal and lipid profile were also obtained.
Results: Patients with DR had significantly longer disease duration and higher systolic blood pressure compared to those without DR. Serum PAI-1 level was significantly higher in patients with DR compared to those without DR, 32.72 (IQR 32.52) vs 21.37 (IQR 14.93) ng/mL, respectively (p < 0.05). However, tear PAI-1 were comparable in both groups. Serum and tear tPA levels in both groups were also comparable (p > 0.05). Among patients with DR, there were no significant correlations between tear and serum of both biomarkers. Patients without DR showed a moderate positive correlation between serum and tear tPA levels with a coefficient of 0.363, albeit no statistical significance. Patients with DR demonstrated a significant positive correlation between levels of tears PAI-1 and BMI (r = 0.555, p = 0.026). In the group without DR, there was a statistically significant positive correlation between serum level of PAI-1 with urine albumin creatinine ratio (UACR) (r = 0.501, p = 0.013).
Conclusion: The present study demonstrated a significantly greater serum PAI-1 levels in patients with DR compared to those without DR. No significant correlations between tears and serum PAI-1 and tPA were observed. Thus, the role of tear biomarkers remains relevant for further investigations.
(© 2022. The Author(s).)
Databáze: MEDLINE
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