Shift in phospholipid and fatty acid contents accompanies brain myelination.

Autor: Naffaa V; Université Paris Cité, CNRS, CiTCoM, F-75006 Paris, France., Magny R; Université Paris Cité, CNRS, CiTCoM, F-75006 Paris, France; Sorbonne Université, UM80, INSERM UMR 968, CNRS UMR 7210, Institut de la Vision, IHU ForeSight, Paris, France., Regazzetti A; Université Paris Cité, CNRS, CiTCoM, F-75006 Paris, France., Van Steenwinckel J; Université Paris Cité, NeuroDiderot, Inserm, F-75019 Paris, France., Gressens P; Université Paris Cité, NeuroDiderot, Inserm, F-75019 Paris, France., Laprévote O; Université Paris Cité, CNRS, CiTCoM, F-75006 Paris, France; Hôpital Européen Georges Pompidou, AP-HP, Service de Biochimie, Paris, France., Auzeil N; Université Paris Cité, CNRS, CiTCoM, F-75006 Paris, France., Schang AL; Université Paris Cité, UMR 1153 CRESS, Paris, France. Electronic address: anne-laure.schang@parisdescartes.fr.
Jazyk: angličtina
Zdroj: Biochimie [Biochimie] 2022 Dec; Vol. 203, pp. 20-31. Date of Electronic Publication: 2022 Aug 30.
DOI: 10.1016/j.biochi.2022.08.010
Abstrakt: In the central nervous system, lipids represent approximately 70% of myelin dry weight and play a key role in axon insulation and action potential conduction velocity. Lipids may thus represent sensitive markers of myelin status in physiological and pathological contexts. In this study, a comprehensive lipidomic analysis by ultra-high-performance liquid chromatography and high-resolution mass spectrometry was performed on myelin-enriched fractions prepared from mouse brains. Two developmental stages were compared: an early rapid myelination stage (postnatal day 15, P15), and a late basal myelination stage (P40). Besides an expected enrichment in characteristic myelin lipids, our study revealed a profound remodeling in phospholipid subclasses during myelination. It included a dramatic decrease in phosphatidylcholine (PC) content and an increase in phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) contents, concomitant to an increased proportion of monounsaturated fatty acids (MUFA) in these subclasses. Lipidomic results were supported by upregulated expression of genes involved in PE, PI, PS and MUFA synthesis in maturing O4+ oligodendrocytes. Highlighted lipid changes may represent key features of brain myelination that could be explored in the context of myelin pathologies.
Competing Interests: Declaration of competing interest All authors have approved the final article.
(Copyright © 2022 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
Databáze: MEDLINE
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