Human ocular surface microcirculation quantified by in vivo computer assisted video microscopy and diffuse reflectance spectroscopy.

Autor: Kvernebo AK; Department of Ophthalmology, Oslo University Hospital, Postboks 4950 Nydalen, 0424, Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, P.O. box 1171 Blindern, 0318, Oslo, Norway; The Circulation Laboratory, Department of Cardiothoracic Surgery, Oslo University Hospital, Postboks 4950 Nydalen, 0424, Oslo, Norway. Electronic address: b24427@ous-hf.no., Moe MC; Department of Ophthalmology, Oslo University Hospital, Postboks 4950 Nydalen, 0424, Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, P.O. box 1171 Blindern, 0318, Oslo, Norway., Wikslund LK; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, P.O. box 1171 Blindern, 0318, Oslo, Norway; The Circulation Laboratory, Department of Cardiothoracic Surgery, Oslo University Hospital, Postboks 4950 Nydalen, 0424, Oslo, Norway; Ostfold Hospital Trust, Gralum, Norway., Capone L; ODI Medical AS, Oslo, Norway., Drolsum L; Department of Ophthalmology, Oslo University Hospital, Postboks 4950 Nydalen, 0424, Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, P.O. box 1171 Blindern, 0318, Oslo, Norway., Kvernebo K; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, P.O. box 1171 Blindern, 0318, Oslo, Norway; The Circulation Laboratory, Department of Cardiothoracic Surgery, Oslo University Hospital, Postboks 4950 Nydalen, 0424, Oslo, Norway; ODI Medical AS, Oslo, Norway.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2022 Nov; Vol. 224, pp. 109232. Date of Electronic Publication: 2022 Aug 31.
DOI: 10.1016/j.exer.2022.109232
Abstrakt: Non-invasive imaging techniques are increasingly used to objectively quantify anterior segment structures of the eye. In this study, we apply the novel oxygen delivery index (ODIN) concept that, quantifies microvascular capacity for oxygen delivery, to the ocular surface in healthy humans. The purpose of the study was to test the applicability of the technologies used for data acquisition from the human ocular surface. We also validated whether the ODIN concept has sufficient sensitivity to detect and differentiate between microvascular structure and function in limbal and bulbar conjunctiva. Multiple ocular surface measurements using computer-assisted video microscopy (field of view: 1.6 mm × 0.9 mm) and diffuse reflectance spectroscopy (measuring volume: ∼0.1 mm3) were obtained from limbal and bulbar conjunctiva in 20 healthy volunteers. Three parameters were extracted during analyses: Functional capillary density, capillary flow velocity, and microvascular oxygen saturation. Functional capillary density was higher at limbus than in bulbar conjunctiva (11.2 ± 1.8 c/mm versus 5.2 ± 1.2 c/mm, p < 0.01), and microvascular oxygen saturation was lower at limbus (77 ± 8%) as compared to bulbar conjunctiva (89 ± 6%), p < 0.01. More than 80% of scored capillaries had continuous blood flow and no difference was seen between the recording sites (p = 0.68). In conclusion, the ODIN concept is applicable for the assessment of human ocular surface microvascular function and has sufficient sensitivity to detect increased capillary density and oxygen extraction at limbus as compared with bulbar conjunctiva.
Competing Interests: Declaration of competing interest The ODIN concept is patented in Japan and USA and is pending Europe. Kvernebo K is founder, shareholder and CMO of ODI Medical AS. ODI Medical AS has provided the analysis algorithms used in this study. Kvernebo AK is related to Kvernebo K. Luigino Capone is currently employed by ODI Medical. Remaining authors have no financial conflicts of interest.
(Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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