| Autor: |
Attallah OA; Department of Chemistry, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt., Nady M; Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Al Gendy AM; Department of Pharmacology, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt., Choucry M; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Chinese Egyptian University, Cairo, Egypt., Khalil MNA; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt. |
| Abstrakt: |
A solid phase extraction (SPE) system for sesquiterpene lactones of damsissa was developed utilising molecularly imprinted polymers (MIPs). The prepared MIPs had a mesoporous structure and particle size of ≈2.65 µm with 3.99 nm pore size. Additionally, MIPs exhibited high thermal stability with degradation temperature between 209 and 459 °C. Optimized MIP-SPE protocol conditions were set at loading step: 1 mL ethanol; washing step: 1 mL water; eluting step: 4 mL methanol. Developed MIP-SPE system showed a binding capacity of 66.66 mg/g based on Langmuir isotherm which was selected as the best fitting model isotherm. Good selectivity coefficients were observed for neoambrosin of 2.37, 1.31 and 1.14 against umbelliferone, quercetin glucoside and p-coumaric acid, respectively. Furthermore, the proposed MIP-SPE protocol displayed some potential in the isolation of sesquiterpene lactones from damsissa plant extract and laid a foundation for the development of more selective MIPs to nonpolar natural products. |
