Detection of Enterobacter cloacae complex strain with a bla NDM-1 -harboring plasmid from an elderly resident at a long-term care facility in Okayama, Japan.

Autor: Gotoh K; Department of Bacteriology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan., Hagiya H; Department of General Medicine, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan. Electronic address: hagiya@okayama-u.ac.jp., Iio K; Microbiology Division, Clinical Laboratory, Okayama University Hospital, Japan., Yamada H; Department of Clinical Laboratory, Okayama City Hospital, Japan., Matsushita O; Department of Bacteriology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan., Otsuka F; Department of General Medicine, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan; Microbiology Division, Clinical Laboratory, Okayama University Hospital, Japan.
Jazyk: angličtina
Zdroj: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy [J Infect Chemother] 2022 Dec; Vol. 28 (12), pp. 1697-1699. Date of Electronic Publication: 2022 Aug 29.
DOI: 10.1016/j.jiac.2022.08.019
Abstrakt: Amidst the global spread of antimicrobial resistance, New Delhi metallo-β-lactamase (NDM)-type carbapenemase-producing Enterobacterales (CPE) remain uncommon in Japan, and the detection of such highly drug-resistant organisms is limited to inbound cases. There is little evidence regarding the prevalence of NDM β-lactamase gene (bla NDM )-harboring CPE in the domestic community, especially in the provincial cities of Japan. Herein, we report the isolation of a bla NDM-1 -harboring plasmid in Enterobacter cloacae complex strain isolated from an elderly woman without a history of traveling abroad who had resided in a long-term care facility in Okayama, Japan. The multidrug-resistant bla NDM -harboring CPE isolate was detected in a stool sample of the patient during routine screening at admission. We performed whole-genome sequencing analysis of the isolate using MiSeq (Illumina) and MinION (Oxford Nanopore Technologies) platforms. The isolate was identified as sequence type 171, which has predominantly been reported in the United States and China. The bla NDM-1 gene was encoded on the 46,161 bp IncX3 plasmid, with sequence similarity to plasmids of similar size isolated from individuals in China. Collectively, the genomic data suggest that an imported CPE isolate may have spread among healthy individuals in the regional area of Japan.
Competing Interests: Declaration of competing interest None to declare.
(Copyright © 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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