Leukocyte Esterase and Nitrite Urine Reagent Strip Utility Under Altered Assay Conditions in Dogs.
| Autor: | Farris J; College of Veterinary Medicine, University of Georgia, Athens, Georgia (J.F.)., Camus MS; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama (M.S.C.)., Krimer PM; From the Athens Veterinary Diagnostic Laboratory (P.M.K.), University of Georgia, Athens, Georgia. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of the American Animal Hospital Association [J Am Anim Hosp Assoc] 2022 Sep 01; Vol. 58 (5), pp. 240-248. |
| DOI: | 10.5326/JAAHA-MS-7233 |
| Abstrakt: | The study evaluated leukocyte esterase (LE) and nitrite reagent pad usefulness in canine urine using multiple time endpoints and decision thresholds. Whole and sedimented urine samples from 116 client-owned dogs were analyzed with reagent strips from four manufacturers. Results for LE and nitrite were recorded every 30 s up to 150 s using "trace" and "+" positive thresholds and compared to microscopic urine sediment analysis and culture results. Sedimented urine assessed at 150 s with a "trace" positive threshold for LE had significantly or trending higher sensitivity (brand dependent) for pyuria detection compared with manufacturer recommendations. Using these parameters, LE sensitivity and negative likelihood ratio were 66.67-89.47%, and 0.13-0.37, respectively. Following manufacturer recommendations, LE specificity and positive likelihood ratio were 96.91-100% and 17.02-30.63, respectively. Nitrite performance for pyuria and bacteriuria detection was poor for all conditions. LE reagent pads are a useful screening test for pyuria detection using sedimented urine and a "trace" positive threshold at 150 s, whereas LE use with traditional manufacturer recommendations is a good confirmatory test for pyuria detection. Nitrite reagent pads are not useful for pyuria or bacteriuria detection. (© 2022 by American Animal Hospital Association.) |
| Databáze: | MEDLINE |
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