Single-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration.

Autor: Wei X; BGI-Hangzhou, Hangzhou 310012, China.; BGI-Shenzhen, Shenzhen 518103, China., Fu S; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou 510631, China., Li H; BGI-Shenzhen, Shenzhen 518103, China.; BGI-Qingdao, Qingdao 266555, China.; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, Qingdao 266555, China., Liu Y; BGI-Shenzhen, Shenzhen 518103, China., Wang S; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China., Feng W; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China., Yang Y; BGI College & Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450000, China., Liu X; BGI-Qingdao, Qingdao 266555, China., Zeng YY; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou 510631, China., Cheng M; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China., Lai Y; Laboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China., Qiu X; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Wu L; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China., Zhang N; BGI-Qingdao, Qingdao 266555, China., Jiang Y; BGI-Shenzhen, Shenzhen 518103, China.; BGI College & Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450000, China., Xu J; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China., Su X; BGI-Qingdao, Qingdao 266555, China., Peng C; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou 510631, China., Han L; BGI-Shenzhen, Shenzhen 518103, China.; Shenzhen Key Laboratory of Single-Cell Omics, BGI-Shenzhen, Shenzhen 518120, China.; Shenzhen Bay Laboratory, Shenzhen 518000, China., Lou WP; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou 510631, China., Liu C; BGI-Shenzhen, Shenzhen 518103, China.; Shenzhen Bay Laboratory, Shenzhen 518000, China., Yuan Y; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China., Ma K; BGI-Shenzhen, Shenzhen 518103, China., Yang T; BGI-Shenzhen, Shenzhen 518103, China., Pan X; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China., Gao S; BGI-Qingdao, Qingdao 266555, China., Chen A; BGI-Shenzhen, Shenzhen 518103, China.; Department of Biology, University of Copenhagen, Copenhagen DK-2200, Denmark., Esteban MA; Laboratory of Integrative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.; Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing 100101, China., Yang H; BGI-Shenzhen, Shenzhen 518103, China.; James D. Watson Institute of Genome Sciences, Hangzhou 310058, China., Wang J; BGI-Shenzhen, Shenzhen 518103, China.; James D. Watson Institute of Genome Sciences, Hangzhou 310058, China., Fan G; BGI-Shenzhen, Shenzhen 518103, China., Liu L; BGI-Hangzhou, Hangzhou 310012, China.; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.; Shenzhen Bay Laboratory, Shenzhen 518000, China., Chen L; Hubei Key Laboratory of Cell Homeostasis, RNA Institute, College of Life Sciences, Wuhan University, Wuhan 430072, China., Xu X; BGI-Shenzhen, Shenzhen 518103, China.; Guangdong Provincial Key Laboratory of Genome Read and Write, BGI-Shenzhen, Shenzhen 518120, China., Fei JF; Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China., Gu Y; BGI-Hangzhou, Hangzhou 310012, China.; BGI-Shenzhen, Shenzhen 518103, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.; Guangdong Provincial Key Laboratory of Genome Read and Write, BGI-Shenzhen, Shenzhen 518120, China.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2022 Sep 02; Vol. 377 (6610), pp. eabp9444. Date of Electronic Publication: 2022 Sep 02.
DOI: 10.1126/science.abp9444
Abstrakt: The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct spatial distribution, molecular features, and functions. We identified an injury-induced ependymoglial cell cluster at the wound site as a progenitor cell population for the potential replenishment of lost neurons, through a cell state transition process resembling neurogenesis during development. Transcriptome comparisons indicated that these induced cells may originate from local resident ependymoglial cells. We further uncovered spatially defined neurons at the lesion site that may regress to an immature neuron-like state. Our work establishes spatial transcriptome profiles of an anamniote tetrapod brain and decodes potential neurogenesis from ependymoglial cells for development and regeneration, thus providing mechanistic insights into vertebrate brain regeneration.
Databáze: MEDLINE
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