Controlling Nanoparticle Uptake in Innate Immune Cells with Heparosan Polysaccharides.

Autor: Yang W; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Frickenstein AN; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Sheth V; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Holden A; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Mettenbrink EM; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Wang L; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Woodward AA; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Joo BS; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Butterfield SK; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Donahue ND; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Green DE; Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma73104, United States., Thomas AG; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Harcourt T; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Young H; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Tang M; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Malik ZA; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Harrison RG; School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, Oklahoma73019, United States., Mukherjee P; Department of Pathology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma73104, United States.; Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma73104, United States., DeAngelis PL; Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma73104, United States., Wilhelm S; Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma73019, United States.; Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma73104, United States.; Institute for Biomedical Engineering, Science, and Technology (IBEST), University of Oklahoma, Norman, Oklahoma73019, United States.
Jazyk: angličtina
Zdroj: Nano letters [Nano Lett] 2022 Sep 14; Vol. 22 (17), pp. 7119-7128. Date of Electronic Publication: 2022 Sep 01.
DOI: 10.1021/acs.nanolett.2c02226
Abstrakt: We used heparosan (HEP) polysaccharides for controlling nanoparticle delivery to innate immune cells. Our results show that HEP-coated nanoparticles were endocytosed in a time-dependent manner by innate immune cells via both clathrin-mediated and macropinocytosis pathways. Upon endocytosis, we observed HEP-coated nanoparticles in intracellular vesicles and the cytoplasm, demonstrating the potential for nanoparticle escape from intracellular vesicles. Competition with other glycosaminoglycan types inhibited the endocytosis of HEP-coated nanoparticles only partially. We further found that nanoparticle uptake into innate immune cells can be controlled by more than 3 orders of magnitude via systematically varying the HEP surface density. Our results suggest a substantial potential for HEP-coated nanoparticles to target innate immune cells for efficient intracellular delivery, including into the cytoplasm. This HEP nanoparticle surface engineering technology may be broadly used to develop efficient nanoscale devices for drug and gene delivery as well as possibly for gene editing and immuno-engineering applications.
Databáze: MEDLINE