| Autor: |
Guo WD; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Hu ZM; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Bu JL; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Wang J; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Ma Y; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Guo J; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Huang LQ; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China. |
| Jazyk: |
čínština |
| Zdroj: |
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2022 Aug; Vol. 47 (16), pp. 4347-4357. |
| DOI: |
10.19540/j.cnki.cjcmm.20220417.102 |
| Abstrakt: |
Paeoniflorin, a representative pinane monoterpene glycoside, is the main active component and quality index of Paeoniae Radix Alba and Paeoniae Radix Rubra.The possible biosynthesis of paeoniflorin is as follows: GPP is derived from mevalonate(MVA) and/or 2-C-methyl-D-erythritol 4-phosphate(MEP) pathway(s) followed by the catalysis with terpene synthase, cytochrome P450(CYP450), UDP-glucuronosyltransferase(UGT), and acyltransferase(AT), respectively.This study aims to explore the genes rela-ted to the biosynthesis of paeoniflorin.To be specific, the cDNA libraries for flowers, leaves, and roots of Paeonia lactiflora were established and sequenced.A total of 30 609 open reading frames(ORFs) were yielded.Through functional annotation and expression analysis of all CYP450 genes in the transcriptome, 11 CYP450 genes belonging to CYP71 A and CYP71 D subfamilies and showing expression trend consistent with monoterpene synthase PlPIN that may be involved in paeoniflorin biosynthesis were screened out.Subsequently, 7 UGT genes and 9 AT genes demonstrating the expression trend consistent with PlPIN which were possibly involved in paeoniflorin biosynthesis were further screened by functional annotation analysis, full-length sequence analysis, expression analysis, and phylogeny analysis.This study provided a systematic screening method with smaller number of candidate genes, thus reducing the workload of functional gene verification.The result laid a foundation for analyzing the biosynthesis pathway of paeoniflorin and the formation mechanism. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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