Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis.

Autor: Akıl C; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, 138673, Singapore.; Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore.; Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama, 700-8530, Japan., Tran LT; Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama, 700-8530, Japan., Orhant-Prioux M; CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054, Grenoble, France., Baskaran Y; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, 138673, Singapore., Senju Y; Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama, 700-8530, Japan., Takeda S; Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama, 700-8530, Japan., Chotchuang P; School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, 21210, Thailand., Muengsaen D; School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, 21210, Thailand., Schulte A; School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, 21210, Thailand., Manser E; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, 138673, Singapore.; Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore., Blanchoin L; CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054, Grenoble, France.; CytomorphoLab, Hôpital Saint Louis, Institut Universitaire d'Hématologie, UMRS1160, INSERM/AP-HP/Université Paris Diderot, 75010, Paris, France., Robinson RC; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, 138673, Singapore. rrobinson@okayama-u.ac.jp.; Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama, 700-8530, Japan. rrobinson@okayama-u.ac.jp.; School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, 21210, Thailand. rrobinson@okayama-u.ac.jp.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2022 Aug 31; Vol. 5 (1), pp. 890. Date of Electronic Publication: 2022 Aug 31.
DOI: 10.1038/s42003-022-03783-1
Abstrakt: Charting the emergence of eukaryotic traits is important for understanding the characteristics of organisms that contributed to eukaryogenesis. Asgard archaea and eukaryotes are the only organisms known to possess regulated actin cytoskeletons. Here, we determined that gelsolins (2DGels) from Lokiarchaeota (Loki) and Heimdallarchaeota (Heim) are capable of regulating eukaryotic actin dynamics in vitro and when expressed in eukaryotic cells. The actin filament severing and capping, and actin monomer sequestering, functionalities of 2DGels are strictly calcium controlled. We determined the X-ray structures of Heim and Loki 2DGels bound actin monomers. Each structure possesses common and distinct calcium-binding sites. Loki2DGel has an unusual WH2-like motif (LVDV) between its two gelsolin domains, in which the aspartic acid coordinates a calcium ion at the interface with actin. We conclude that the calcium-regulated actin cytoskeleton predates eukaryogenesis and emerged in the predecessors of the last common ancestor of Loki, Heim and Thorarchaeota.
(© 2022. The Author(s).)
Databáze: MEDLINE
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