Endothelial versus pronephron fate decision is modulated by the transcription factors Cloche/Npas4l, Tal1, and Lmo2.

Autor:	Mattonet K; Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany.; DZHK (German Center for Cardiovascular Research), partner site, 43, D-61231 Bad Nauheim.; CPI (Cardio Pulmonary Institute), partner site, 43, D-61231 Bad Nauheim.; DZL (German Center for Lung Research), partner site, 43, D-61231 Bad Nauheim., Riemslagh FW; Section of Developmental Biology, Department of Pediatrics, University of Colorado School of Medicine, Anschutz Medical Campus, 12801 E 17th Avenue, Aurora, CO 80045, USA., Guenther S; DZHK (German Center for Cardiovascular Research), partner site, 43, D-61231 Bad Nauheim.; CPI (Cardio Pulmonary Institute), partner site, 43, D-61231 Bad Nauheim.; Bioinformatics and Deep Sequencing Platform, Max Planck Institute for Heart and Lung Research, Bad Nauheim 61231, Germany., Prummel KD; Section of Developmental Biology, Department of Pediatrics, University of Colorado School of Medicine, Anschutz Medical Campus, 12801 E 17th Avenue, Aurora, CO 80045, USA., Kesavan G; Center for Regenerative Therapies at TU Dresden (CRTD); Dresden, Germany., Hans S; Center for Regenerative Therapies at TU Dresden (CRTD); Dresden, Germany., Ebersberger I; Goethe University Frankfurt am Main, Institute of Cell Biology and Neuroscience, Frankfurt 60438, Germany.; Senckenberg Biodiversity and Climate Research Center (S-BIKF), Frankfurt 60325, Germany.; LOEWE Center for Translational Biodiversity Genomics (TBG), Frankfurt 60325, Germany., Brand M; Center for Regenerative Therapies at TU Dresden (CRTD); Dresden, Germany., Burger A; Section of Developmental Biology, Department of Pediatrics, University of Colorado School of Medicine, Anschutz Medical Campus, 12801 E 17th Avenue, Aurora, CO 80045, USA., Reischauer S; Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany.; CPI (Cardio Pulmonary Institute), partner site, 43, D-61231 Bad Nauheim., Mosimann C; Section of Developmental Biology, Department of Pediatrics, University of Colorado School of Medicine, Anschutz Medical Campus, 12801 E 17th Avenue, Aurora, CO 80045, USA., Stainier DYR; Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231, Germany.; DZHK (German Center for Cardiovascular Research), partner site, 43, D-61231 Bad Nauheim.; CPI (Cardio Pulmonary Institute), partner site, 43, D-61231 Bad Nauheim.; DZL (German Center for Lung Research), partner site, 43, D-61231 Bad Nauheim.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2022 Sep 02; Vol. 8 (35), pp. eabn2082. Date of Electronic Publication: 2022 Aug 31.
DOI:	10.1126/sciadv.abn2082
Abstrakt:	Endothelial specification is a key event during embryogenesis; however, when, and how, endothelial cells separate from other lineages is poorly understood. In zebrafish, Npas4l is indispensable for endothelial specification by inducing the expression of the transcription factor genes etsrp , tal1 , and lmo2 . We generated a knock-in reporter in zebrafish npas4l to visualize endothelial progenitors and their derivatives in wild-type and mutant embryos. Unexpectedly, we find that in npas4l mutants, npas4l reporter-expressing cells contribute to the pronephron tubules. Single-cell transcriptomics and live imaging of the early lateral plate mesoderm in wild-type embryos indeed reveals coexpression of endothelial and pronephron markers, a finding confirmed by creERT2-based lineage tracing. Increased contribution of npas4l reporter-expressing cells to pronephron tubules is also observed in tal1 and lmo2 mutants and is reversed in npas4l mutants injected with tal1 mRNA. Together, these data reveal that Npas4l/Tal1/Lmo2 regulate the fate decision between the endothelial and pronephron lineages.
Databáze:	MEDLINE
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