Determination of gibberellic acid and abscisic acid in ( Zea mays L.) (ICA-V305) seeds germinated using dynamic sonication assisted solvent extraction and maceration.

Autor: Rivera JD; Departamento de Química, Grupo de Estudios Ambientales en Agua y Suelo, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia., Ocampo-Serna DM; Departamento de Química, Grupo de Estudios Ambientales en Agua y Suelo, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia., Martínez-Rubio RA; Departamento de Química, Grupo de Estudios Ambientales en Agua y Suelo, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia., Correa-Navarro YM; Departamento de Química, Grupo de Estudios Ambientales en Agua y Suelo, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.
Jazyk: angličtina
Zdroj: MethodsX [MethodsX] 2022 Aug 13; Vol. 9, pp. 101821. Date of Electronic Publication: 2022 Aug 13 (Print Publication: 2022).
DOI: 10.1016/j.mex.2022.101821
Abstrakt: Phytohormones are molecules responsible for growth, development, and metabolism regulation in plants. Gibberellic acid (GA 3 ) and abscisic acid (ABA) are the main phytohormones involved in seed germination. Notably, it should be a highlight that GA 3 induces germination, whereas ABA inhibits it. For this reason, it is important to calculate the concentration of these two phytohormones during seeds germination. Firstly, the maize seeds (MS) were germinated and samples of these were taken at different imbibition times, after that, methanol extracts were obtained using two methods of dynamic solvent extraction assisted by sonication (DSASE) and a traditional extraction method (maceration); finally, to estimate the concentration of GA 3 and ABA a high performance liquid chromatographic method was used. The results of this study showed that the three extraction methods used, allowed quantifying GA 3 and ABA during the maize germination time studied. However, of the two extraction methods employed, DSASE was the best technique because higher concentrations of GA 3 and ABA were found. Therefore, it is important to continue using this green chemistry methodology for these and other analyses.•The extraction protocol developed was based on dynamic sonication-assisted solvent extraction.•The chromatographic method used allowed the simultaneous determination of two phytohormones with different physicochemical properties in maize seeds.•This methodology offers good sensitivity, linearity, precision, reproducibility and suitable detection and quantification limits.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2022 The Author(s). Published by Elsevier B.V.)
Databáze: MEDLINE
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