Morin attenuates neurobehavioural deficits, hippocampal oxidative stress, inflammation, and apoptosis in rats co-exposed to bisphenol S and diethyl phthalate.

Autor: Ugwor EI; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria. Electronic address: ugworei@funaab.edu.ng., Dosumu OA; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Eteng OE; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Moses CA; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Ogbonna CU; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., James AS; Department of Chemical Sciences, Faculty of Science, Augustine University, Ilara-Epe P.M.B 1100, Lagos State, Nigeria., Adeleye AO; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Ilavbarhe ME; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Ajasa F; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Olawuyi O; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria., Ugbaja RN; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta P.M.B. 2240, Ogun State, Nigeria.
Jazyk: angličtina
Zdroj: Brain research [Brain Res] 2022 Nov 01; Vol. 1794, pp. 148068. Date of Electronic Publication: 2022 Aug 27.
DOI: 10.1016/j.brainres.2022.148068
Abstrakt: Endocrine-disrupting pollutants (EDPs) remain pervasive in the environment. Bisphenol S (BPS) and diethyl phthalates (DEP) are commonly used to replace the more toxic EDPs. However, it is unclear if they induce neurotoxicity, like their predecessors. Morin possesses relevant neuro-pharmacological activities. Hence, we sought to evaluate the protective effects of morin against the neurotoxic effects previously reported for EDPs. Male Wistar rats were exposed to a mixture of BPS and DEP (MBD) and treated with morin for 21 days. Behavioural assessments were conducted, and the hippocampal tissues were processed for analysis. Rats exposed to MBD presented anxiety-like behaviours, impaired cognitive and motor functions compared to the control group. MBD exposure induced hyperactivity of neurosignalling enzymes (AChE, ADA, MAO-A) and depleted hippocampal antioxidants (SOD, CAT, GPx, and GSH). MBD exposure increased calcium levels and inhibited total Ca 2+ -ATPase activity. Levels of reactive species (NO and H 2 O 2 ) and oxidative damage markers (MDA and AOPP) were significantly (P < 0.05) elevated compared to control. The hippocampal expressions of IL-1β, TNFα, BAX, and APAF-1 in the MBD-exposed rats were significantly higher compared to control. Correspondingly, NF-κB and caspase-3 pathways were activated in the hippocampus of MBD-exposed rats, while the expressions of IL-10 and BDNF were repressed. However, co-treatment with morin improved the neurobehavioral outcomes, alleviated the hyperactivity of neurosignalling enzymes, while suppressing hippocampal oxidative stress, inflammation, and apoptosis. Histological and stereological evaluations supported these findings. In conclusion, co-exposure to BPS and DEP elicit similar neurotoxic outcomes as their predecessors, while morin confers marked protection against these outcomes.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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