Bioaffinity-based surface immobilization of antibodies to capture endothelial colony-forming cells.

Autor: Boulanger MD; Department of Chemical Engineering, McGill University, Montreal, Canada., Level HA; Department of Chemical Engineering, McGill University, Montreal, Canada., Elkhodiry MA; Department of Chemical Engineering, McGill University, Montreal, Canada., Bashth OS; Department of Chemical Engineering, McGill University, Montreal, Canada., Chevallier P; Laboratoire d'Ingénierie de Surface, Centre de Recherche sur les Matériaux Avancés, Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada.; Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, Québec, Canada., Laroche G; Laboratoire d'Ingénierie de Surface, Centre de Recherche sur les Matériaux Avancés, Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada.; Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, Québec, Canada., Hoesli CA; Department of Chemical Engineering, McGill University, Montreal, Canada.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Aug 30; Vol. 17 (8), pp. e0269316. Date of Electronic Publication: 2022 Aug 30 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0269316
Abstrakt: Maximizing the re-endothelialization of vascular implants such as prostheses or stents has the potential to significantly improve their long-term performance. Endothelial progenitor cell capture stents with surface-immobilized antibodies show significantly improved endothelialization in the clinic. However, most current antibody-based stent surface modification strategies rely on antibody adsorption or direct conjugation via amino or carboxyl groups which leads to poor control over antibody surface concentration and/or molecular orientation, and ultimately bioavailability for cell capture. Here, we assess the utility of a bioaffinity-based surface modification strategy to immobilize antibodies targeting endothelial cell surface antigens. A cysteine-tagged truncated protein G polypeptide containing three Fc-binding domains was conjugated onto aminated polystyrene substrates via a bi-functional linking arm, followed by antibody immobilization. Different IgG antibodies were successfully immobilized on the protein G-modified surfaces. Covalent grafting of the protein G polypeptide was more effective than surface adsorption in immobilizing antibodies at high density based on fluorophore-labeled secondary antibody detection, as well as endothelial colony-forming cell capture through anti-CD144 antibodies. This work presents a potential avenue for enhancing the performance of cell capture strategies by using covalent grafting of protein G polypeptides to immobilize IgG antibodies.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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