Transcriptomic, Proteomic, and Morphologic Characterization of Healing in Volumetric Muscle Loss.

Autor: Crum RJ; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Johnson SA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Jiang P; Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, Ohio, USA.; Center for Applied Data Analysis and Modeling (ADAM), Cleveland State University, Cleveland, Ohio, USA.; Department of Biological, Geological, and Environmental Sciences (BGES), Cleveland State University, Cleveland, Ohio, USA., Jui JH; Department of Computer Science, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Zamora R; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Center for Inflammation and Regeneration Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Center for Systems Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Cortes D; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Kulkarni M; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Prabahar A; Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, Ohio, USA., Bolin J; Morgridge Institute for Research, Madison, Wisconsin, USA., Gann E; Department of Surgery, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA.; Surgical Critical Care Initiative, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA., Elster E; Department of Surgery, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA.; Surgical Critical Care Initiative, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA.; Walter Reed National Military Medical Center, Bethesda, Maryland, USA., Schobel SA; Department of Surgery, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA.; Surgical Critical Care Initiative, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA., Larie D; Department of Surgery, University of Vermont, Burlington, Vermont, USA., Cockrell C; Department of Surgery, University of Vermont, Burlington, Vermont, USA., An G; Department of Surgery, University of Vermont, Burlington, Vermont, USA., Brown B; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Hauskrecht M; Department of Computer Science, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Vodovotz Y; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Center for Inflammation and Regeneration Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Center for Systems Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Badylak SF; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Jazyk: angličtina
Zdroj: Tissue engineering. Part A [Tissue Eng Part A] 2022 Dec; Vol. 28 (23-24), pp. 941-957. Date of Electronic Publication: 2022 Oct 11.
DOI: 10.1089/ten.TEA.2022.0113
Abstrakt: Skeletal muscle has a robust, inherent ability to regenerate in response to injury from acute to chronic. In severe trauma, however, complete regeneration is not possible, resulting in a permanent loss of skeletal muscle tissue referred to as volumetric muscle loss (VML). There are few consistently reliable therapeutic or surgical options to address VML. A major limitation in investigation of possible therapies is the absence of a well-characterized large animal model. In this study, we present results of a comprehensive transcriptomic, proteomic, and morphologic characterization of wound healing following VML in a novel canine model of VML which we compare to a nine-patient cohort of combat-associated VML. The canine model is translationally relevant as it provides both a regional (spatial) and temporal map of the wound healing processes that occur in human VML. Collectively, these data show the spatiotemporal transcriptomic, proteomic, and morphologic properties of canine VML healing as a framework and model system applicable to future studies investigating novel therapies for human VML. Impact Statement The spatiotemporal transcriptomic, proteomic, and morphologic properties of canine volumetric muscle loss (VML) healing is a translational framework and model system applicable to future studies investigating novel therapies for human VML.
Databáze: MEDLINE