Xiaotansanjiefang inhibits the viability of colorectal cancer cells via Jagged 1/Notch 3/Snail signaling pathway.

Autor: Ye M; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Du J; Department of Bai's Proctology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Wang X; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Xiu L; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Liu X; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Gu Y; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Pei B; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Sun D; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China., Yue X; Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Jazyk: angličtina
Zdroj: Environmental toxicology [Environ Toxicol] 2022 Dec; Vol. 37 (12), pp. 2957-2964. Date of Electronic Publication: 2022 Aug 30.
DOI: 10.1002/tox.23651
Abstrakt: The purpose of this study is to explore the anti-colorectal cancer of Xiaotansanjiefang, a famous traditional Chinese medicine, and its potential anti-cancer mechanism. In this study, the HCT116 cell spheres were prepared as in vitro study model. We found the Xiaotansanjiefang medication was able to inhibit the proliferation of HCT116 cell spheres in a dose-dependent manner, especially in 3 and 6 mg/ml Xiaotansanjiefang medication treated groups. We also found the high concentration of Xiaotansanjiefang medication could suppress the migration and promote the apoptosis of HCT116 cell spheres. Moreover, we found the expression of Jagged 1, Notch 3, Snail, and Hes 1 were decreased in HCT116 cell spheres treated with Xiaotansanjiefang medication. Furthermore, the proliferation and apoptosis behaviors of HCT116 cell spheres treated with Xiaotansanjiefang medication were reversed with the addition of Jagged 1 Fc chimera protein. The expression of Jagged 1, Notch 3, Snail, and Hes 1 were also increased again in HCT116 cells treated with Xiaotansanjiefang medication plus with Jagged 1 Fc chimera protein. The presented study may provide a promising strategy to treat and prevent colorectal cancer.
(© 2022 The Authors. Environmental Toxicology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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