Pathomorphology, immunohistochemical, and molecular detection of an atypical porcine dermatitis and nephropathy syndrome (PDNS) due to PCV-2d-2 in naturally affected grower pigs of India.

Autor: Sahoo M; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India. Electronic address: vety.lisa@gmail.com., Pathak M; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India., Patel SK; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India; Department of Veterinary Pathology, College of Veterinary Science & Animal Husbandry, Rewa, Madhya Pradesh, India., Saikumar G; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India. Electronic address: saikumarivri@gmail.com., Upmanyu V; Division of Biological Standardization, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India., Thakor JC; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India., Kumar P; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India., Singh R; Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India; Veterinary Pathology, Institute of Veterinary Science and Animal Husbandry, Siksha 'O' Anusandhan, Ghatikia, Bhubaneswar, Odisha, India., Singh K; ICAR-CADRAD, Indian Veterinary Research Institute (IVRI), Izatnagar, India., Sahoo NR; ICAR-DFMD-International Centre for Foot and Mouth Disease, Arugul, Jatni, Bhubaneswar, Odisha, India.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2022 Oct; Vol. 171, pp. 105738. Date of Electronic Publication: 2022 Aug 26.
DOI: 10.1016/j.micpath.2022.105738
Abstrakt: Porcine circovirus type 2 (PCV-2) is of great economic significance to porcine industry worldwide. PCV-2 variants and genotypes, alternating world over, are the etiological agent of several clinical syndromes such as porcine dermatitis and nephropathy syndrome (PDNS), post-weaning multi-systemic wasting syndrome (PMWS) and others in pigs. This study is reporting an atypical manifestation of PDNS in twelve grower pigs, 3- to - 4.5 months age and either sex, died of the disease, with predominant lesions of nephropathy and no obvious clinical lesions in skin. Necropsy examination of pigs showed lesions of petechial -to- ecchymotic hemorrhages in the kidneys and in the right auricular musculature of the hearts. Microscopic lesions in H & E sections of the kidneys showed acute glomerulonephritis, interstitial nephritis, and vasculitis, but the skin morphology and architecture remained unaltered in contrast to the pathognomonic lesions of PDNS described in the literature. Other syndromic associations of PDNS in these cases included-perimyocarditis, interstitial pneumonia, depleted lymphoid tissues, tonsillitis, enteritis, and meningo-encephalitis. The lesional sites in duplicate paraffin tissue sections of kidneys, heart, lungs, spleen, lymph nodes, intestine, and brain demonstrated PCV-2 antigen in the cytoplasm of cells as highlighted by the intense immunolabeling on IHC staining. The PCV-2 positive organs reconfirmed by PCR, targeting ORF2 gene, which yielded 481bp size of products. The sequencing results of 481bp products on phylogenetic analysis showed 94% similarity with that of PCV-2 sequences in the database that grouped into PCV2d-2 genotype. The present report confirms, probably for the first time, the atypical PDNS cases due to PCV2d-2 genotype in naturally affected grower pigs of India.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Monalisa Sahoo reports financial support was provided by Indian Veterinary Research Institute. Monalisa Sahoo reports a relationship with Indian Veterinary Research Institute that includes: employment. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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