Radio-iodination and biological evaluation of pentoxifylline as a novel probe for diagnosis of intermittent claudication.

Autor: Motaleb MA; Labeled Compounds Department, Hot Lab Center, Egyptian Atomic Energy Authority, 11371, Cairo, Egypt., Shweeta HA; Labeled Compounds Department, Hot Lab Center, Egyptian Atomic Energy Authority, 11371, Cairo, Egypt., Aboumanei MH; Labeled Compounds Department, Hot Lab Center, Egyptian Atomic Energy Authority, 11371, Cairo, Egypt. Electronic address: Dr.mohamedaboumanei@gmail.com.
Jazyk: angličtina
Zdroj: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2022 Nov; Vol. 189, pp. 110429. Date of Electronic Publication: 2022 Aug 24.
DOI: 10.1016/j.apradiso.2022.110429
Abstrakt: The diagnosis of intermittent claudication (IC) is challenging. Imaging with radiopharmaceuticals provides a new method for detecting acute IC. Pentoxifylline improves blood flow to ischemic tissues via increasing erythrocyte elasticity and inhibiting platelet aggregation. Pentoxifylline was radio-iodinated with radioiodine-131 ( 131 I) through a direct electrophilic substitution reaction. Furthermore, various factors that might influence the radiolabeling strategy were investigated. The radiochemical yield of [ 131 I]iodopentoxiphyline was evaluated by using paper chromatography and HPLC methods. The biodistribution pattern of [ 131 I]iodopentoxiphyline was studied, where Swiss albino mice was used as a model of acute limb ischemia-reperfusion. The maximum radiochemical yield of pentoxifylline was found to be 94.11 ± 2.35%. The biodistribution findings revealed that [ 131 I]iodopentoxiphyline was significantly deposited at the ischemic site (left hind limbs), with encouraging target/non-target (T/NT) ratios. At 0.25 and 1 h post injection, the uptake of [ 131 I]iodo-pentoxifylline was 5.30 ± 0.30 and 9.98 ± 1.12%, respectively. Also, the maximum T/NT ratio for [ 131 I]iodo-pentoxifylline (9.45 ± 0.26) was obtained at 0.25 h post injection. Due to safety and selectivity, [ 131 I]iodo-pentoxifylline may be a good prospective diagnostic tool for early identification of IC. Moreover, the outcome of this study can be expected to apply to I-123 as well.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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