Involvement of caspase-3 in apoptosis of human lymphocytes exposed to cadmium chloride.
| Autor: | Mirkamali M; Biology Department, Faculty of Science, 125649Arak University, Arak, Iran., Momeni HR; Biology Department, Faculty of Science, 125649Arak University, Arak, Iran., Etemadi T; Biology Department, Faculty of Science, 125649Arak University, Arak, Iran., Mosayebi G; Department of Microbiology and Immunology, Molecular and Medicine Research Center (MMRC), 48412Arak University of Medical Sciences, Arak, Iran., Komijani M; Biology Department, Faculty of Science, 125649Arak University, Arak, Iran. |
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| Jazyk: | angličtina |
| Zdroj: | Human & experimental toxicology [Hum Exp Toxicol] 2022 Jan-Dec; Vol. 41, pp. 9603271221121796. |
| DOI: | 10.1177/09603271221121796 |
| Abstrakt: | Background: Lymphocytes are a group of white blood cells with a variety of roles their integrity is crucial for the body's immune responses. Cadmium, a heavy metal and environmental pollutant, is known as a toxicant to exert its adverse effects on some sort of cells including blood cells. Research Design: In this study, human lymphocytes were divided into 3 groups: (1) lymphocytes at 0-h, (2) lymphocytes at 24 h (control), (3) lymphocytes treated with cadmium chloride (15 μM). Lymphocyte viability and plasma membrane integrity were assessed in these groups. In addition, the occurrence of apoptosis was investigated by assessment of nucleus diameter and flow cytometry. Activation of caspase-3 was also detected by immunocytochemistry. Results: Result showed that lymphocyte's viability and plasma membrane integrity decreased in lymphocytes treated with cadmium as compared with the control group. Decreased nucleus diameter and result of flow cytometry demonstrated cadmium-induced apoptosis in human lymphocytes. Furthermore, lymphocytes treated with cadmium displayed intensely activated caspase-3 immunoreactivity in their cytoplasm. Conclusion: In conclusion, cadmium not only negatively effect on viability and plasma membrane, but also induces caspase-dependent apoptosis in human lymphocytes. |
| Databáze: | MEDLINE |
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